| 固定化辣根过氧化物酶的电化学和电催化特性 |
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| 引用本文： | 李业梅,张卫东.固定化辣根过氧化物酶的电化学和电催化特性[J].化学世界,2008,49(9). |
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| 作者姓名： | 李业梅 张卫东 |
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| 作者单位： | 郧阳师范高等专科学校化学系,湖北,丹江口,442700 |
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| 摘 要： | 用海藻酸钠(SA)将辣根过氧化物酶(HRP)固定到热裂解石墨电极表面,制备了HRP-SA膜修饰电极。研究发现包埋在SA膜中的辣根过氧化物酶可与电极直接传递电子,在缓冲溶液中可得到一对辣根过氧化物酶辅基血红素Fe(Ⅲ)/Fe(Ⅱ)电对的可逆氧化还原峰。其式电势随溶液pH值增加而负移,且呈线性关系,这说明辣根过氧化物酶的电子传递过程伴随有质子的转移。并考察了HRP-SA膜修饰电极对氧气、双氧水、一氧化氮的电催化性质。
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| 关 键 词： | 辣根过氧化物酶 海藻酸钠 直接电化学 电催化 |
Electrochemical and Electrocatalysisl Behavior of Horseradish Peroxidase Entrapped in Sodium Alginate Hydrogel Films |
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| LI Ye-mei,ZHANG Wei-dong.Electrochemical and Electrocatalysisl Behavior of Horseradish Peroxidase Entrapped in Sodium Alginate Hydrogel Films[J].Chemical World,2008,49(9). |
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| Authors: | LI Ye-mei ZHANG Wei-dong |
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| Abstract: | Horseradish peroxidase was immobilized on edge-plane pyrolytic graphite(EPG) electrode by sodium alginate hydrogel.The horseradish peroxidase entrapped in the sodium alginate film underweat fast direct electron-transfer reaction.The formal potential(E~(o′)) was linearly dependent on solution pH,indicating that the electron transfer was proton-coupled.The catalytic reduction of oxygen, hydrogen peroxide and nitric oxide by the horseradish peroxidase entrapped in sodium alginate hydrogel was also explored. |
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| Keywords: | horseradish peroxidase sodium alginate electrochemistry electrocatalysis |
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