Evaluation of structural,mechanical, and cellular behavior of electrospun poly-3-hydroxybutyrate scaffolds loaded with glucosamine sulfate to develop cartilage tissue engineering |
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| Authors: | Zahra Shahali Mohammad Reza Avadi Dariush Semnani Elham Naghash Zargar Batoul HashemiBeni |
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| Affiliation: | 1. Department of Medical Nanotechnology, Faculty of Advanced Sciences &2. Technology, Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran;3. Department of Textile Engineering, Isfahan University of Technology, Isfahan, Iran;4. Department of Anatomical Sciences and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran |
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| Abstract: | Considering the role of glucosamine sulfate (GS) in the biosynthetic pathways of chondrocytes, an attempt was made to design an electrospun poly-3-hydroxybutyrate (PHB) scaffold loaded with GS to develop cartilage tissue engineering. The study was initiated using the optimal electrospun scaffold conditions for the synthesis of PHB/GS. The resulting scaffolds have shown excellent pore architectures and mechanical behavior compared to pure PHB. UV spectrophotometric analysis, for the evaluation of the GS release behavior, showed zero-order kinetics release. In vitro results indicated excellent cell viability, cell adhesion, and cell penetration of PHB/GS scaffolds compared to pure PHB. |
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| Keywords: | Cartilage tissue engineering chondrocyte electrospun scaffold glucosamine sulfate poly-3-hydroxybutyrate |
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