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Poly(n-butyl methacrylate) with primary amine end groups for supporting cell adhesion and proliferation of renal epithelial cells |
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| Authors: | Kayleigh Cox-Nowak Ohood Al-Yamani Colin A Grant Nicola H Green |
| Affiliation: | 1. Polymer and Biomaterials Chemistry Laboratories, University of Sheffield, Sheffield, UK;2. School of Chemistry and Biociences, University of Bradford, Bradford, UK;3. Advanced Materials Engineering, Polymer IRC Labs, Faculty of Engineering and Informatics, University of Bradford, Bradford, UK;4. Materials Science and Engineering, Kroto Research Institute, University of Sheffield, Sheffield, UK |
| Abstract: | Polymer coatings that support epithelial cell culture have been developed. Ozonolysis and subsequent workup of poly(butyl methacrylate-co-butadiene) copolymers is used to form oligomers with carboxylic acid end groups, which are then further reacted with diamines to provide poly(butyl methacrylate)s with primary amine end groups. The polymers are cast as films and used as cell culture substrates for human dermal fibroblasts and human renal epithelial cells. Fibroblast and epithelial cells adhere and proliferate on acid functional materials but on amine functional films epithelial cells show greater viability than fibroblasts. |
| Keywords: | Amines biocoatings epithelial cells fibroblast cells |