New N-(2-carboxybenzyl)chitosan composite scaffolds containing nanoTiO2 or bioactive glass with enhanced cell proliferation for bone-tissue engineering applications |
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Authors: | Maria C Nerantzaki Iro G Koliakou Martha G Kaloyianni Zoi N Terzopoulou Evangelia K Siska Michalis A Karakassides |
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Affiliation: | 1. Laboratory of Polymer Chemistry and Technology, Department of Chemistry, Aristotle University of Thessaloniki, Thessaloniki, Greece;2. Laboratory of Animal Physiology, Department of Biology, University of Thessaloniki, Thessaloniki, Greece;3. Laboratory of Biochemistry, Faculty of Medicine, Aristotle University of Thessaloniki, Thessaloniki, Greece;4. Laboratory of Ceramics and Composite Materials, Department of Materials Science and Engineering, University of Ioannina, Ioannina, Greece |
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Abstract: | In the present study N-(2-carboxbenzyl)chitosan (CBCS) 3D macroporous hybrid scaffolds with interconnected pore system, containing 0.5, 2.5, and 5?wt% TiO2 nanoparticles (nTiO2) and 2.5?wt% Bioglass 45S5 (BG) have been synthesized using freeze-drying technique. Compressive strength values verified that the modification of chitosan combined with the presence of inorganic fillers can attribute significant mechanical stiffness to the scaffold. The in vitro biomineralization test confirmed that all samples were bioinert as mineral deposits were detected with X-ray diffractometry after incubation in SBF. Cytotoxicity and biocompatibility of all scaffolds were tested by using and Wharton’s jelly–derived mesenchymal stem cells (WJ-MSCs) and human embryonic kidney 293 (HEK 293) cell line. Metabolic activity, proliferation, migration, and attachment to the scaffolds were examined. Cells appeared to attach around the superficial pores and migrate in them. Cells also maintained their morphology, proliferated, and migrated across the scaffolds and showed consistent and proved compatibility. |
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Keywords: | N-(2-Carboxybenzyl)chitosan bioglass 45S5 titania nanoparticles composites scaffolds cells biocompatibility |
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