Synthesis strategies for optimizing sizes of PLGA nanoparticles containing recombinant Chenopodium album (rChe a 3) allergen |
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| Authors: | Jafar Hajavi Mojtaba Sankian Abdol-Reza Varasteh |
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| Affiliation: | 1. Student Research Committee, Mashhad University of Medical Sciences, Mashhad, Iran;2. Immunology Research Center, Medical School, Mashhad University of Medical Sciences, Mashhad, Iran;3. Immunology Research Center, Medical School, Mashhad University of Medical Sciences, Mashhad, Iran;4. Allergy Research Center, Medical School, Mashhad University of Medical Sciences, Mashhad, Iran |
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| Abstract: | The size of antigen delivery systems impact on the immune induction. The purpose of this study was to obtain poly(lactide-co-glycolide) (PLGA) nanoparticles (NPs) containing rChe a 3 allergen in different sizes (about 200, 400, and 800?nm). The optimal conditions were obtained by adjusting different parameters comprising molecular weight/concentration of polyvinyl alcohol, solvent type (dichloromethane or dichloromethane/acetone), sonication time, and addition of cryoprotectant (sucrose). The optimized formulations were found to be stable for 60 days at 4°C. In conclusion, the preparation conditions need to be adjusted to obtain allergen-PLGA NPs with specific aforementioned sizes. |
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| Keywords: | Allergen encapsulation efficiency PLGA preparation conditions size |
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