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Head-to-Head Comparison of Selected Extra- and Intracellular CO-Releasing Molecules on Their CO-Releasing and Anti-Inflammatory Properties |
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| Authors: | Yingchun Li Lars Hemmersbach Bernhard Krause Dr. Nikolay Sitnikov Dr. Anna Schlundt née Göderz Diego O. Pastene Maldonado Prof. Dr. Hans-Günther Schmalz Prof. Dr. Benito Yard |
| Affiliation: | 1. Vth medical Department, Medical Faculty Mannheim, University of Heidelberg, Theodor-Kutzer-Ufer 1–3, 68167 Mannheim, Germany These authors contributed equally to this work.;2. Universität zu Köln, Greinstrasse 4, 50939 Köln, Germany These authors contributed equally to this work.;3. Universität zu Köln, Greinstrasse 4, 50939 Köln, Germany;4. Vth medical Department, Medical Faculty Mannheim, University of Heidelberg, Theodor-Kutzer-Ufer 1–3, 68167 Mannheim, Germany |
| Abstract: | Over the past decade, a variety of carbon monoxide releasing molecules (CORMs) have been developed and tested. Some CORMs spontaneously release CO once in solution, while others require a trigger mechanism to release the bound CO from its molecular complex. The modulation of biological systems by CORMs depends largely on the spatiotemporal release of CO, which likely differs among the different types of CORMs. In spontaneously releasing CORMs, CO is released extracellularly and crosses the cell membrane to interact with intracellular targets. Other CORMs can directly release CO intracellularly, which may be a more efficient method to modulate biological systems. In the present study, we compared the efficacy of extracellular and intracellular CO-releasing CORMs that either release CO spontaneously or require an enzymatic trigger. The efficacy of such CORMs to modulate HO-1 and VCAM-1 expression in TNF-α-stimulated human umbilical vein endothelial cells (HUVEC) was evaluated. |
| Keywords: | anti-inflammatory agents heme oxygenases iron carbonyl complexes prodrugs carbon monoxide |