|
|||||
|
|
Host-Assisted Delivery of a Model Drug to Genomic DNA: Key Information from Ultrafast Spectroscopy and in silico Study |
|
| Authors: | Ria Ghosh Soumendra Singh Dipanjan Mukherjee Susmita Mondal Monojit Das Uttam Pal Aniruddha Adhikari Aman Bhushan Surajit Bose Siddharth Sankar Bhattacharyya Debasish Pal Tanusri Saha-Dasgupta Maitree Bhattacharyya Debasis Bhattacharyya Asim Kumar Mallick Ranjan Das Samir Kumar Pal |
| Affiliation: | 1. Department of Chemical Biological and Macromolecular Sciences, S.N. Bose National Centre for Basic Sciences, Block JD, Sector 3, Salt lake, Kolkata 700106 India
Department of Biochemistry, University of Calcutta, 35 Ballygunge Circular Rd, Ballygunge, Kolkata 700019 India;2. Technical Research Centre, S. N. Bose National Centre for Basic Sciences, Block JD, Sector 3, Salt Lake, Kolkata 700106 India;3. Department of Chemical Biological and Macromolecular Sciences, S.N. Bose National Centre for Basic Sciences, Block JD, Sector 3, Salt lake, Kolkata 700106 India;4. Department of Zoology, Uluberia College, University of Calcutta, Howrah, 711315 India Department of Zoology, Vidyasagar University, Midnapore, 721102 India;5. Department of Biotechnology, Thapar Institute of Engineering and Technology, Bhadson Road, Patiala, Punjab 147004 India;6. Department of Oral and Maxillofacial Pathology, KSDJ Dental College and Hospital, 700002 Kolkata, India;7. Department of Zoology, Uluberia College, University of Calcutta, Howrah, 711315 India;8. Technical Research Centre, S. N. Bose National Centre for Basic Sciences, Block JD, Sector 3, Salt Lake, Kolkata 700106 India Department of Condensed Matter Physics and Material Sciences, S. N. Bose National Centre for Basic Sciences, Kolkata, 700106 India;9. Department of Biochemistry, University of Calcutta, 35 Ballygunge Circular Rd, Ballygunge, Kolkata 700019 India;10. Department of Gynecology & Obstetrics, Nil Ratan Sircar Medical College & Hospital, 138, AJC Bose Road, Sealdah, Raja Bazar, Kolkata, 700014 India;11. Department of Pediatric Medicine, Nil Ratan Sirkar Medical College and Hospital, Kolkata, 700014 India;12. Department of Chemistry, West Bengal State University, Barasat, 700126 Kolkata India |
| Abstract: | Drug delivery to a target without adverse effects is one of the major criteria for clinical use. Herein, we have made an attempt to explore the delivery efficacy of SDS surfactant in a monomer and micellar stage during the delivery of the model drug, Toluidine Blue (TB) from the micellar cavity to DNA. Molecular recognition of pre-micellar SDS encapsulated TB with DNA occurs at a rate constant of k1 ～652 s?1. However, no significant release of encapsulated TB at micellar concentration was observed within the experimental time frame. This originated from the higher binding affinity of TB towards the nano-cavity of SDS at micellar concentration which does not allow the delivery of TB from the nano-cavity of SDS micelles to DNA. Thus, molecular recognition controls the extent of DNA recognition by TB which in turn modulates the rate of delivery of TB from SDS in a concentration-dependent manner. |
| Keywords: | DNA drug delivery micelles pre-micelles toluidine blue |