| Affiliation: | 1. Key Laboratory of Flexible Electronics (KLOFE) &, Institute of Advanced Materials (IAM), Nanjing Tech University (NanjingTech), Nanjing, 211800 China These authors contributed equally to this work.;2. Frontiers Science Center for Flexible Electronics, Xi'an Institute of Flexible Electronics (IFE) and, Xi'an Institute of Biomedical Materials & Engineering, Northwestern Polytechnical University, Xi'an, 710072 China;3. Key Laboratory of Flexible Electronics (KLOFE) &, Institute of Advanced Materials (IAM), Nanjing Tech University (NanjingTech), Nanjing, 211800 China;4. The Institute of Flexible Electronics (IFE, Future Technologies), Xiamen University, Xiamen, 361005 Fujian, China;5. School of Basic Medical Sciences, Shanxi Medical University, Taiyuan, 310003 China |
| Abstract: | Impaired mitophagy hinders the clearance of damaged mitochondria, inducing pathological states. Knowledge of this phenomenon is key to diagnosing certain diseases and understanding their pathogenesis. Mitophagy involves an acidization process that could serve as an ideal detection target. In this work, we designed and synthesized a mitochondrial-targeting fluorescence probe, Z2 , for evaluating pH variation. This probe exhibited remarkable “turn-on” fluorescence under acidic conditions. In biological applications, Z2 showed a strong, specific pH detection capacity in Parkin-overexpressing HeLa cells during the mitophagy process. The “turn-on” fluorescence property of Z2 was also used to detect pH variations in Caenorhabditis elegans. This probe, as a novel pH assessment tool, may facilitate further research of mitophagy-associated pathological patterns. |
