Biosynthetic Gene Cluster of Linaridin Peptides Contains Epimerase Gene |
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Authors: | Wanlu Xiao Dr. Yasuharu Satoh Dr. Yasushi Ogasawara Prof. Dr. Tohru Dairi |
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Affiliation: | 1. Graduate School of Chemical Sciences and Engineering, Hokkaido University, N13 W8, Kita-ku Sapporo, Hokkaido, 060-8628 Japan;2. Graduate School of Engineering, Hokkaido University, N13 W8, Kita-ku Sapporo, Hokkaido, 060-8628 Japan |
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Abstract: | Salinipeptins belong to the type-A linaridin class of ribosomally synthesized and post-translationally modified peptides (RiPPs) comprising 22 amino acid residues with multiple D -amino acids. Although chirality of other type-A linaridins, such as grisemycin and cypemycin, has not been reported, the biosynthetic gene clusters of type-A linaridins have identical gene organization. Here, we report heterologous expression of grisemycin biosynthetic gene cluster (grm) and show that grisemycin contains multiple D -amino acids, similar to salinipeptins. The heterologous expression experiments also confirm the involvement of a novel peptide epimerase in grisemycin biosynthesis. Gene-deletion experiments indicate that grmL, a single gene with unknown function, is indispensable for grisemycin production. We also show that the presence of D -amino acids is likely a common feature of linaridin natural products by analyzing two other type-A linaridin clusters. |
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Keywords: | biosynthesis D-amino acids epimerases linaridin peptides |
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