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Sulfation Pattern Dependent Iron(III) Mediated Interleukin-8 Glycan Binding |
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| Authors: | Francesco Brunori Dr. Deepak Kumar Padhi Israel Alshanski Dr. Joanna Freyse Jan-Niklas Dürig Anja Penk Prof. Luigi Vaccaro Dr. Mattan Hurevich Prof. Jörg Rademann Prof. Shlomo Yitzchaik |
| Affiliation: | 1. Institute of Chemistry and Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem, Safra Campus, Givat Ram, Jerusalem, 91904 Israel Laboratory of Green Synthetic Organic Chemistry, Dipartimento di Chimica, Biologiae Biotecnologie, Università di Perugia, Via Elce di Sotto 8, 06123 Perugia, Italy These authors contributed equally to this work.;2. Institute of Chemistry and Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem, Safra Campus, Givat Ram, Jerusalem, 91904 Israel These authors contributed equally to this work.;3. Institute of Chemistry and Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem, Safra Campus, Givat Ram, Jerusalem, 91904 Israel;4. Medicinal Chemistry, Freie Universität Berlin, Königin-Luise-Strasse 2+4, Berlin, 14195 Germany;5. Institute of Medical Physics and Biophysics, Leipzig University, Medical Faculty, Härtelstraße 16/18, 04107 Leipzig, Germany;6. Laboratory of Green Synthetic Organic Chemistry, Dipartimento di Chimica, Biologiae Biotecnologie, Università di Perugia, Via Elce di Sotto 8, 06123 Perugia, Italy |
| Abstract: | Cytokines such as interleukin-8 activate the immune system during infection and interact with sulfated glycosaminoglycans with specific sulfation patterns. In some cases, these interactions are mediated by metal ion binding which can be used to tune surface-based glycan-protein interactions. We evaluated the effect of both hyaluronan sulfation degree and Fe3+ on interleukin-8 binding by electrochemical impedance spectroscopy and surface characterizations. Our results show that sulfation degree and metal ion interactions have a synergistic effect in tuning the electrochemical response of the glycated surfaces to the cytokine. |
| Keywords: | electrochemistry interactions interleukin-8 metal binding sulfation pattern |