HPLC-ICP-MS法测定水样中的甲基汞、乙基汞和无机汞

建立了高效液相色谱(HPLC)和电感耦合等离子体质谱(ICP-MS)联用测定环境水样中的甲基汞、乙基汞和无机汞的方法。实验使用的高效液相色谱流动相为含有0.06mol/L乙酸氨,20μg/LBi,0.1%(V/V)2-巯基乙醇的5%(V/V)甲醇-水溶液,色谱柱为C18反相柱(5μm,2.1mm×50mm),经前处理的水样在液相色谱中分离后,进入电感耦合等离子体质谱检测其甲基汞、乙基汞和无机汞的浓度。甲基汞、乙基汞和无机汞检出限分别为0.05μg/L、0.10μg/L和0.10μg/L。

Simultaneous determination of the content of Methyl mercury,Ethyl mercury and Inorganic mercury in water samples by HPLC-ICP-MS

A confirmatory method is presented for the determination of Methyl mercury, Ethyl mercury and inorganic mercury in environmental water samples by high performance liquid chromatography coupled with inductively coupled plasma-mass spectrometry （HPLC-ICP-MS）. A 5%（V/V） methanol-water mobile phase containing 0.06 mol/L ammonium acetate, 10μg/L Bi and 0.1%（V/V ） 2-mercaptoethanol and a C18 reversed phase colmnn （5μm, 2.1 mm × 150 mm） was used for separation of Methyl mercury, Ethyl mercury and inorganic mercury by HPLC. The concentrations of three Hg species in eluent were detected by ICP-MS, The limits of the method were about 0.05μg/L, 0.10μg/L and 0.10μg/L for Methyl mercury, Ethyl mercury and inorganic mercury respectively.