HPLC法测定肉制品中苯甲酸、山梨酸、脱氢乙酸

建立用高效液相色谱二极管阵列检测器测定肉制品中苯甲酸、山梨酸、脱氢乙酸的方法。肉制品中苯甲酸、山梨酸、脱氢乙酸经水蒸气蒸馏,用高效液相色谱二极管阵列检测器测定,外标峰面积法定量。苯甲酸、山梨酸、脱氢乙酸在2~100 mg/kg范围内呈良好的线性关系,3个水平(2.0、20.0、80.0 mg/kg)添加苯甲酸、山梨酸、脱氢乙酸的回收率为84.3%~91.1%,相对标准偏差(RSD)为1.04%~2.06%,检出限为2.0mg/kg。此方法具有简单、快速、准确等优点,适用于脂肪含量较高的肉制品中苯甲酸、山梨酸、脱氢乙酸的测定。

HPLC Determination of Benzoic Acid,Sorbic Acid and Dehydroacetic Acid in Meat and Products

High efficiency liquid chromatography- diode array detector method was developed for the determination of Benzoic acid, Sorbic acid and Dehydroacetic acid in mest and Products. The whole determination procedure consisted of steam distillation, HPLC analysis, and quantitation by external standard method. The developed method showed a good linearity over the wide range of 2-100 mg/kg. Average recoveries of Benzoic acid, Sorbic acid and Dehydroacetic acid in spiked samples at three levels（2.0, 20.0, 80.0 mg/kg） varied from 84.3 % to 91. 1%, with relative standard deviations between 1.04 % and 2.06 %. The limit of detection was 2 mg/kg. Therefore, this method, thanks to its simplicity and rapidity, has a good applicability to the determination of Benzoic acid, Sorbic acid and Dehydroacetic acid in fatty meat and products.