柄篮状菌与里氏木霉酶解棕榈纤维效果的比较

以青霉属柄篮状菌和里氏木霉Rut-C30为研究对象,在50 IU/g底物酶用量的条件下,比较研究了这两种菌株各自对棕榈空果串纤维的酶解能力。结果表明,柄篮状菌对纤维素的酶解能好于Rut-C30,96 h后,两者葡萄糖转化率为83.7%、61.7%。Rut-C30对木聚糖的酶解能力相对较好,其木糖转化率为98.3%,而柄篮状菌酶解的木糖转化率仅为33.1%。将两菌株的酶按1∶1酶用量混合后,棕榈纤维的整体水解效果增强,葡萄糖、木糖转化率依次为91.3%和98.1%。该研究为柄篮状菌与木霉混合水解棕榈纤维提供了依据。

Compared Cellulase Hydrolytic Performance of Oil Palm Empty Fruit Bunches between Talaromyces Stipitatus and Trichoderma Reesei

Hydrolysis of oil pahn empty fruit bunches （OPEFB） was conducted using cellulase from the penicillium strain Talaromyces stipitatus and Trichoderma reesei Rut-C30 with the enzyme loading of 50 IU/g biomass. Results showed that 7~ stipitatus celtulase performed better capability of cellulose hydrolysis than T. reesei Rut C30 cellutase. After 96 h, the corresponding glucose conversion rate was 83.7 % and 61.7 %, respectively. Whereas, for xylan hydrolysis, T. reesei Rut-C30 cellulase performed better with xylose conversion rate of 98.3 % for T. reesei Rut-C30 cellulase and 33.1% for T. stipitatus cellulase. When both cellulases were mixed in an enzyme loading ratio of I ： 1, the overall efficiency of OPEFB hydrolysis was enhanced with respective 91.3 % and 98.1% conversion rate for glucose and xylose. The work provided some evidences for mixed hydrolysis of OPEFB by T stipitatus and T, reesei cellulose.