高效液相色谱法测定化妆品中维生素A,维生素A醋酸酯,维生素A棕榈酸酯

建立了一种测定化妆品中3种维生素A类化合物的分析方法。样品经乙醇直接超声提取后经反相C8柱(4.6×250 mm,5μm)分离,甲醇-水作为流动相,梯度洗脱,流速为1.0 mL/min,二极管阵列检测器进行检测,检测波长为325 nm,外标法定量。在优化实验条件下,3种维生素A类化合物在0.2~5.0 mg/L范围内线性关系良好,在空白化妆品中的加标回收率为96.2%~100%,相对标准偏差为0.3%~4.0%。维生素A,维生素A醋酸酯的方法检出限(S/N=3)为1.0 mg/kg,维生素A棕榈酸酯的方法检出限(S/N=3)为3.0 mg/kg。该方法简单、快速、准确,可满足化妆品中3种维生素A类化合物的检测要求。

Determination of Vitamin A、 Vitamin A acetate and Vitamin A Palmitate in Cosmetics by HPLC-DAD

A method was developed for the determination of 3 Vitamin A compounds in cosmetics. The sample was extracted by ethanol and separated by a RP Ca chromatographic column （4.6×250 mm, 5 μm） at a flow rate of 1.0 mL/min. Methanol-water was used as mobile phase. The detection was performed at 325 nm by using high performance liquid chromatography-diode array detection （HPLC-DAD）. The external standard method was employed for quantitative analysis. Under the optimized condition, the calibration curve was linear in the range of 0.2-5.0 mg/L. The average recoveries of spiked samples were between 96.2 % and 100 %, with relative standard deviations of 0.3 %-4.0 %. The results showed that the limits of detection （LODs, S/N=3） of Vitamin A, Vitamin A acetate and Vitamin A palmitate was separately 1.0, 1.0 and 3.0 mg/kg. The method was simple, rapid, and accurate and could meet the requirement for determination of 3 Vitamin A compounds in cosmetics.