| HPLC测定山葛降脂滴丸中葛根素的含量 |
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| 引用本文： | 郭嘉俊,冯月兴,宋凤兰.HPLC测定山葛降脂滴丸中葛根素的含量[J].广东化工,2014(17):165-166. |
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| 作者姓名： | 郭嘉俊 冯月兴 宋凤兰 |
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| 作者单位： | 广东药学院,广东中山528458 |
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| 基金项目： | 2013年国家级大学生创新创业训练计划项目（201310573014） |
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| 摘 要： | 目的采用高效液相色谱法(HPLC)对山葛降脂滴丸中葛根素含量进行测定。方法色谱柱为Diaomonsil C18色谱柱(250×4.6 mm,5μm)、ODS预柱,以甲醇-0.2%磷酸溶液(28∶72),检测波长为250 nm。结果葛根素在11.75~188.0μg/mL内,浓度与相应峰面积呈良好的线性关系(r=0.9999),平均回收率为99.50%,RSD为1.44%。结论该方法对山葛降脂滴丸中葛根素含量的测定简便易行,结果准确可靠,适用于对山葛降脂滴丸进行质量控制。
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| 关 键 词： | HPLC 山葛降脂滴丸 葛根素 含量测定 |
Determination of Puerarin Content in Shange Lipid-lowering Dropping Pills by HPLC |
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| Guo Jiajun,Feng Yuexing,Song Fenglan.Determination of Puerarin Content in Shange Lipid-lowering Dropping Pills by HPLC[J].Guangdong Chemical Industry,2014(17):165-166. |
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| Authors: | Guo Jiajun Feng Yuexing Song Fenglan |
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| Affiliation: | (Guangdong Pharmaceutical University, Zhongshan 528458, China) |
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| Abstract: | Objective The content of puerarin in shange lipid-lowering dripping pills had been determined by HPLC method. Methods The chromatographic colunm was Diaomonsil Ci8（250×4.6 mm, 5 μm）, ODS pre-column. Mobile phase is methanol -0.2 % orthophosphoric acid（28 ： 72）, the detection wave-length was 250 nm. Results The good linear detection range of puerarin was 11.75-188.0 μg/mL. The recovery of puerarina was 99.50 % （RSD=1.44 %）. Conclusion The present method for determining content of puerarin in shange lipid-lowering dripping pills is simple, feasible with accurate and reliable results, which is suitable for the quality control of this preparation. |
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| Keywords: | HPLC： shange lipid-lowering dripping pills puerarin： assay |
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