HPLC测定逍遥颗粒中的芍药苷

目的:测定不同批次逍遥颗粒中的芍药苷的含量。方法:采用HPLC法,色谱柱为Agilent TC C18(250 mm×4.6 mm,5μm);流动相为乙腈-1%磷酸溶液(20∶80);检测波长为230 nm;柱温为30℃;流速为1.0 ml/min;进样体积10μL。结果:芍药苷1.35~27.00μg·mL-1与峰面积呈良好的线性关系(r=0.999),平均加样回收率为98.55%,RSD=1.8%(n=6);5批次逍遥颗粒中的芍药苷的含量为0.32%。结论:所用方法简单、稳定,芍药苷的含量可作为逍遥颗粒的品质评价依据之一。

Determination of Paeoniflorin in Xiaoyao Particles by HPLC

Objective： To determine the content of Paeoniflorin in the Xiaoyao Particles of different batches. Methods： HPLC analysis was adopted with the column ofa Agilent TC C18 （250 mm × 4.6 mm, 5 μm） at 30 ℃ of column temperature. The mobile phase was acetonitrile -1% phosphate （20 ： 80） with detection wavelength of 230 nm and flow rate was 1.0 mLmin-I. The injection volume was 10 μL. Results： Paeoniflorin showed a good linear relationship between area integration value and sampling amount at a range of 1.35-27.00 μg ml-1 （r=0.999）, with the average recovery of 98.55 % and RSD = 1.8 % （n = 6）. The mean content of Paeoniflorin in 5 batches of Xiaoyao Particles was 0.32 %. Conclusion： The method is simple and stable, which can be used as one of the bases of the quality cvaluation of Xiaoyao Particles.