| 同位素质谱联用技术鉴别无蛋白蜂蜜的真实性 |
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| 引用本文： | 罗东辉,罗海英,冼燕萍,王斌,吴文海,陈意光,郭新东,吴玉銮.同位素质谱联用技术鉴别无蛋白蜂蜜的真实性[J].广州食品工业科技,2012(7):862-866. |
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| 作者姓名： | 罗东辉 罗海英 冼燕萍 王斌 吴文海 陈意光 郭新东 吴玉銮 |
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| 作者单位： | 广州市质量监督检测研究院,国家加工食品质量监督检验中心（广州）,广州市食品安全检测技术重点实验室,广州市食品安全风险动态监测与预警研究中心,广东广州510110 |
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| 基金项目： | 广州市科技计划项目（[2011]233-34：2011J2200016）;广东省质量技术监督局科技项目（2011ZS01） |
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| 摘 要： | 本文从120份蜂蜜样品中筛选出15份无蛋白蜂蜜样品，应用元素分析-同位素质谱联用技术（EA-IRMS）、液相色谱-同位素质谱联用技术（LC-IRMS）、液相色谱测定蜂蜜还原糖含量等检测方法，进行无蛋白蜂蜜样品真实性系统研究。发现15份无蛋白样品皆为掺假掺杂蜂蜜，无蛋白蜂蜜样品的三类典型掺假掺杂手段为：一是掺入碳-4植物源水解产物；二是掺入碳-3植物源水解产物；三是以碳．3植物源高纯度果葡糖浆造假。
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| 关 键 词： | 蜂蜜 蛋白 同位素质谱 掺假掺杂 |
Identification the Authenticity of no Protein Honey by IRMS |
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| Authors: | LUO Dong-hui LUO Hai-ying XIAN Yan-ping WANG Bin WU Wen-hai CHEN Yi-guang GUO Xin-dong WU Yu-luan |
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| Affiliation: | (Guangzhou Quality Supervision and Testing Institute, National Centre for Quality Supervision and Testing of Processed Food (Guangzhou), Guangzhou City Key Laboratory of Detection Technology for Food Safety, Guangzhou City Research Center of Risk Dynamic Detection and Early Warning for Food Safety, Guangzhou Guangdong 510110, China) |
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| Abstract: | Fifteen honey samples which found to contain no protein fi＇om 120 specimens were further investigated by element analyzer-isotope ratio mass spectrometry, liquid chromatography-isotope ratio mass spectrometry and liquid chromatography, the protein components in the honey samples were detected. It was found that adulteration existed in all the tested fifteen samples. The samples were found to be adulterated by C-4 plant hydrolyzate, C-3 plant hydrolyzate or C-3 plant high fructose corn syrup. |
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| Keywords: | honey protein isotope ratio mass spectrometry （IRMS） adulteration |
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