微波辅助萃取花椰菜废弃菜叶叶绿素的工艺研究

应用微波辐射技术，以花椰菜废弃菜叶为原料提取叶绿素。以菜叶粉碎目数、破壁助剂浓度、微波功率、辐照时间、萃取剂浓度、料液比作为影响因素进行单因素试验，分析确定单因素的最佳实验条件。采用L16（4’）正交试验设计，优化菜叶叶绿素的提取条件。结果表明：微波辅助萃取菜叶叶绿素的最佳提取条件为破壁助剂乙醇浓度60％，辐照时间30s，萃取剂乙醇浓度90％，料液比1：20，叶绿素的提取率可以达到6．84mg／g，较传统提取方法的提取率提高62．2％。

Microwave Assisted Extraction of Cauliflower Trash Foliage Chlorophyll

Cauliflower foliage was used as the raw material for extracting chlorophyll with microwave extraction. Some factors were considered for single factor experiment, such as size of cauliflower foliage powder, density of cell wall crack agent, microwave power, irradiation time, extractant concentration, solid-liquid ratio. L16（45） orthogonal experiment was adopted for getting the optimal mixed test conditions. The result showed that the extraction ratio of chlorophyll could be achieved as 6.843mg/g under the following conditions： 60% of density of cell wall crack agent, 30s of irradiation time, 90% of extractant concentration and 1：20 of solid-liquid ratio. Compared with the traditional technology, microwave assisted extraction method can increase extracting rate of chlorophyll by 62.2%.