氧化镍-脂肪酶修饰丝网印刷电极测定二油酸甘油酯

利用电化学氧化沉积纳米氧化镍（Nio）于丝网印刷电极（SPE），使用壳聚糖包埋法将脂肪酶固定在NiO-SPE上构建一种新的脂肪酶生物传感器用于测定二油酸甘油酯。以铁氰化钾作为电子媒介体，在1％Triton-X100，PBS（0．1mol／L，pn7．0）体系中，用循环伏安法（CV）表征了二油酸甘油酯在Lipase-NiO．SPE上的伏安行为，并用CV检测二油酸甘油酯。在0．04-180μmol／L范围内呈现良好线性关系（R=0．993），检出限为0．01μmol／L。该方法简单、快速、准备、稳定，可用于二油酸甘油酯含量的检测。

Detection of Dioleglyceride Using NiO-lipase Modified Screen-printed Electrode

A new enzyme sensor for determination of dioleglyceride was constructed by nano-niclde oxide modified through electro- chemical oxidation sedimentary reaction on the screen-printied electrode （SPE） and lipase immobilized by chitosan. Using ferricyanide as the electron mediator,, the CV behavior of dioleglyceride on lipase-NiO-SPE were evalutaed in the solution of 1% Triton-X100 and PBS buffer （0.1 mol/L, pH 7.0）. A good linear relationship was obtained between dioleglyceride ranging in 0.04-180μmol/L with cyclic voltamrnetry （R =0.993） and detection limit of 0.01μmol/L. This method was simple, rapid, accurate, and steady for the determination of dioleglyceride.