猪粪中四环素类抗生素残留物的生物降解

通过L9(34)正交批量实验，研究了温度、初始含水率、时间对猪粪中土霉素、四环素和金霉素生物降解的影响，并考察了此过程中微生物群落的变化. 结果表明，3种四环素类抗生素在55.0℃、初始含水率60.0%时降解14 d的降解率最大，均符合一级反应动力学模型，且细菌为优势微生物. 统计分析表明，温度是土霉素和四环素降解的主要影响因素，初始含水率是金霉素降解的主要影响因素，但上述因素对3种四环素类抗生素的降解率及真菌、放线菌和细菌的相对丰度均没有显著性影响.

Biodegradation of Tetracycline Antibiotics Residues in Swine Manure

L9(34) orthogonal experiments by simulating the aerated static pile process were carried out to investigate the biodegradation of oxytetracycline (OTC), tetracycline (TC) and chlortetracycline (CTC) residues in swine manure at different incubation temperatures (35.0, 45.0 and 55.0℃), initial moisture contents (40.0%, 60.0% and 75.0%) and time (7, 14 and 21 d). Meanwhile, the microbial community of total heterotrophic microorganisms was analyzed by standard dilution plating of incubation at 28, 45 and 55℃, respectively, for 4 d. The results showed that the biodegradation rates of these three tetracyclines (TCs) would reach the maximum at the conditions of 55.0℃, 60.0% and 14 d. The biodegradation of TCs in swine manure met with the first order kinetics model. Statistical analysis showed that temperature was the major factor of OTC and TC biodegradation, and initial moisture content was the major factor of CTC biodegradation, however, these three factors did not have a significant effect on the biodegradation rate of these TCs and relative ratio of fungi, actinomycete and bacteria, respectively. The bacteria were the predominance during the biodegradation of TCs residue in swine manure.