Prolift材质医用网片在家兔体内的生物相容性及缩变的实验研究

目的探讨Prolift材质医用网片在动物体内的生物相容性及面积缩变度。方法将6只新西兰家兔按随机数字表法分为3组：A组、B组和C组,每组2只。每只家兔腹部皮下放置10 mm×40 mm Prolift材质医用网片10片。3组家兔术后均采用氟哌酸50 mg.d-1加入饲料中口服,抗感染3 d。A组中1只家兔出现感染为A1组,1只未感染的家兔为A2组;B、C 2组家兔均未出现感染。A组术后第14天处死家兔,B组术后第30天处死家兔,C组术后第90天处死家兔,各组家兔分别取出Prolift材质医用网片及肌肉组织,以家兔自身的皮下组织及肌肉组织作为对照组。用游标卡尺测量Prolift材质医用网片的长、宽值,计算面积及Prolift材质医用网片面积缩变度。同时行组织切片,在光学显微镜下观察各组家兔的炎症反应、纤维组织囊壁形成情况。结果 A1组家兔术后第3天出现植入Prolift材质医用网片部位感染,各组家兔均无血清肿、侵蚀、暴露及排斥反应等。A1、A2、B、C 4组家兔Prolift材质医用网片面积缩变度分别为（21.84±0.05）%、（3.13±0.03）%、（2.30±0.03）%、（2.50±0.03）%,A1组家兔Prolift材质医用网片面积缩变度和处死后的网片面积与A2组比较差异均有统计学意义（均P〈0.05）,家兔Prolift材质医用网片面积缩变度和处死后的网片面积A2组、B组、C组之间比较差异均无统计学意义（均P〉0.05）。A2、B、C 3组家兔炎症反应分级分别为Ⅲ级、Ⅱ级、〈Ⅰ级,纤维组织囊壁形成分别为Ⅲ级、Ⅱ级、〈Ⅰ级。结论 Prolift材质医用网片植入家兔体内后具有良好的抗缩变性能和生物相容性,但感染可能是引起网片缩变的重要因素。

Biocompatibility and Shrinkage of Prolift Mesh in Rabbits in Vivo

Objective To explore the biocompatibility and area shrinkage degree of Prolift mesh in rabbits in vivo.Methods Six New Zealand rabbits were randomly divided into three groups：group A,group B and group C,with 2 rabbits in each group.Ten pieces of Prolift mesh（size 10 mm×40 mm） were implanted in the subcutaneous tissue of the abdomen.All rabbits received oral norfloxacin 50 mg·d-1for 3 days postoperatively.The infected rabbit in group A was considered as group A1,and the other one as group A2.No infection was observed in group B and group C.Prolift mesh material and muscular tissues were isolated on the 14,30 and 90th postoperative day in group A,group B and group C,respectively.The rabbit＇s own subcutaneous and muscular tissues were obtained as the control.The values of the length and width of the Prolift mesh were measured with vernier caliper.The mesh area and area shrinkage degree were calculated.Tissue sections were evaluated histologically for the inflammatory response and fibrous tissue cyst formation by light microscopy.Results The implant site infection occurred in group A1 on the third postoperative day.There were no seromas,erosion,exposure or rejection reactions in all rabbits.The area shrinkage degree of Prolift mesh material in group A1,A2,B and C was（21.84±0.05）%,（3.13±0.03）%,（2.30%±0.03）% and（2.50±0.03）%,respectively.There were significant differences in the area shrinkage degree and mesh area between group A1 and A2（all P0.05）,but no differences were observed among group A2,B and C（all P0.05）.Inflammatory grading was grade Ⅲ,gradeⅡ and gradeⅠ in group A2,B and C,respectively,and fibrous tissue capsule formation was classified as grade Ⅲ,gradeⅡ and grade Ⅰ,respectively.ConclusionProlift mesh material exhibits good anti-shrinkage properties and excellent biocompatibility after implantation in rabbits in vivo.However,the infection may be an important factor leading to mesh area shrinkage degree.