| 抗马铃薯Y病毒(PVY)和烟草花叶病毒(TMV)单联弱毒疫苗的研制及防效测定 |
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| 引用本文： | 姜瀚林,田延平,郭兆奎,刘永中,万秀清,刘文涛,李现道,李向东,张永春,孟凡武.抗马铃薯Y病毒(PVY)和烟草花叶病毒(TMV)单联弱毒疫苗的研制及防效测定[J].中国烟草学报,2020,26(2):65-70. |
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| 作者姓名： | 姜瀚林 田延平 郭兆奎 刘永中 万秀清 刘文涛 李现道 李向东 张永春 孟凡武 |
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| 作者单位： | 1.山东农业大学植物保护学院, 山东泰安岱宗大街61号 271018 |
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| 基金项目： | 黑龙江省烟草公司项目(HN201703),山东省烟草公司项目(KN238-201602),泰山学者建设工程(TS201712023)。 |
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| 摘 要： | 为防治烟草马铃薯Y病毒（Potato virus Y，PVY）和烟草花叶病毒（Tobacco mosaic virus，TMV），筛选PVY和TMV基因组中保守且高效表达siRNA的片段，连接到烟草脉带花叶病毒（Tobacco vein banding mosaic virus，TVBMV）弱毒侵染性克隆，制备成单联弱毒疫苗，获得TVB-PVYF1、TVB-PVYF2、TVB-PVYF3和TVB-TMVF1、TVB-TMVF2、TVB-TMVF3共6个单联弱毒疫苗。室内筛选后选取保护效果最好的TVB-PVYF2和TVB-TMVF3两个单联弱毒疫苗在山东临沂、潍坊和黑龙江哈尔滨等地进行了田间防效测定，TVB-PVYF2对黑龙江哈尔滨、山东临沂和潍坊烟草PVY的相对防效分别为66.88%、76.46%和60.71%，TVB-TMVF3对黑龙江哈尔滨、山东临沂和潍坊烟草TMV的相对防效分别为80.74%、87.34%和74.40%。
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| 关 键 词： | 马铃薯Y病毒 烟草花叶病毒 RNA沉默 弱毒疫苗 |
| 收稿时间： | 2019-07-21 |
Preparation and control effect determination of mild vaccines against Potato virus Y and Tobacco mosaic virus |
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| JIANG Hanlin,TIAN Yanping,GUO Zhaokui,LIU Yongzhou,WAN Xiuqing,LIU Wentao,LI Xiandao,LI Xiangdong,ZHANG Yongchun,MENG Fanwu.Preparation and control effect determination of mild vaccines against Potato virus Y and Tobacco mosaic virus[J].Acta Tabacaria Sinica,2020,26(2):65-70. |
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| Authors: | JIANG Hanlin TIAN Yanping GUO Zhaokui LIU Yongzhou WAN Xiuqing LIU Wentao LI Xiandao LI Xiangdong ZHANG Yongchun MENG Fanwu |
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| Affiliation: | 1.College of Plant Protection, Shandong Agricultural University, Tai'an 271018, Shandong, China2.Heilongjiang Tobacco Research Institute, Harbin 1570076, China3.Linyi Municipal Tobacco Company, Linyi 276003, Shandong, China4.Shandong Provincial Tobacco Academy, Ji'nan 250098, China5.Weifang Municipal Tobacco Company, Weifang 261061, Shandong, China |
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| Abstract: | To control Potato virus Y virus (PVY) and Tobacco mosaic virus (TMV),the conserved genomic sequences of PVY and TMV that have a great potential to produce siRNAs were screened and cloned to mild mutant vector of Tobacco vein banding mosaic virus (TVBMV),producing mild vaccines including TVB-PVYF1,TVB-PVYF2,TVB-PVYF3 and TVB-TMVF1,TVB-TMVF2,TVB-TMVF3.Primary tests showed that TVB-PVYF2 and TVB-TMVF3 had great potential on cross protection.Field tests were conducted in Harbin of Heilongjiang province,Linyi and Weifang of Shandong province.The results showed that relative control effects of TVB-PVYF2 against PVY in Harbin,Linyi and Weifang were 66.88%,76.46% and 60.71%,respectively,while those of TVB-TMVF3 against TMV in Harbin,Linyi and Weifang were 80.74%,87.34% and 74.40%,respectively. |
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| Keywords: | Potato virus Y Tobacco mosaic virus RNA silence mild vaccine |
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