| 三株芽孢杆菌的生防特性研究 |
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| 引用本文： | 李苗苗,张家韬,崔传斌,孙光军,王东坤,崔志燕,高强,冯超,王凤龙,陈德鑫,王晓强. 三株芽孢杆菌的生防特性研究[J]. 中国烟草科学, 2020, 41(4): 80-89. DOI: 10.13496/j.issn.1007-5119.2020.04.013 |
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| 作者姓名： | 李苗苗 张家韬 崔传斌 孙光军 王东坤 崔志燕 高强 冯超 王凤龙 陈德鑫 王晓强 |
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| 作者单位： | 1. 中国农业科学院烟草研究所, 青岛 266000;2. 中国烟草总公司陕西省烟草公司, 西安 712000;3. 中国烟草总公司贵州省公司, 贵阳 550004;4. 陕西省烟草公司商洛市公司, 陕西 商洛 726000;5. 山东省烟草公司临沂市公司, 山东 临沂 276002;6. 中国烟草总公司海南省公司, 海口 571199 |
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| 基金项目： | 山东省自然科学基金项目“吡咯伯克霍尔德氏菌Lyc2抗细菌相关基因的鉴定与功能分析”（ZR2018BC037）；陕西省烟草公司项目“陕西烟草病虫害绿色防控技术研究示范与推广应用”（SXYC-KJ-2017-01）；贵州省烟草公司科技项目“贵州烟草叶斑类病害成灾规律与绿色防控技术研究示范”（201920）；陕西省商洛市烟草公司项目“商洛烟草主要叶部病害绿色防控技术研究与示范推广”（2019-ZY-1） |
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| 摘 要： | 为明确贝莱斯芽孢杆菌（Bacillus velezensis）GY1、解淀粉芽孢杆菌（Bacillus amyloliquefaciens）GY10和枯草芽孢杆菌（Bacillus subtilis）GY12的生防特性，采用平板试验法、对扣法、盆栽试验等，对三株生防菌的抑菌谱、次生代谢产物、挥发性物质、根际定殖能力和促生能力等进行了研究。结果表明，GY1、GY10、GY12培养液可以显著促进烟草种子的萌发和烟株的生长；三株生防菌抑菌谱广，对烟草赤星病菌、黑胫病菌及花生镰刀菌等9种病原具有显著的抑制作用，并且都具有分泌嗜铁素和蛋白酶的能力；三株生防菌发酵上清液和挥发性物质对链格孢菌（Alternaria alternata）和烟草疫霉菌（Phytophthora parasitica var.nicotianae）具有显著抑制作用；菌株的分泌蛋白热稳定性好，对烟草赤星病菌具有显著的体外抑菌活性，并对酸性环境具有较高的耐受性；根际定殖结果表明，GY1-GY10-GY12复配与单菌株相比能增加各生防菌在烟草根际的定殖。以上结果对于菌株GY1、GY10、GY12生防特性的深入挖掘和产业化开发具有一定的指导作用。
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| 关 键 词： | 芽孢杆菌 烟草黑胫病 烟草赤星病 生防特性 根际定殖 |
| 收稿时间： | 2020-03-10 |
Study on Biocontrol Characteristics of Three Bacillus Strains |
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| LI Miaomiao,ZHANG Jiatao,CUI Chuanbin,SUN Guangjun,WANG Dongkun,CUI Zhiyan,GAO Qiang,FENG Chao,WANG Fenglong,CHEN Dexin,WANG Xiaoqiang. Study on Biocontrol Characteristics of Three Bacillus Strains[J]. Chinese Tobacco Science, 2020, 41(4): 80-89. DOI: 10.13496/j.issn.1007-5119.2020.04.013 |
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| Authors: | LI Miaomiao ZHANG Jiatao CUI Chuanbin SUN Guangjun WANG Dongkun CUI Zhiyan GAO Qiang FENG Chao WANG Fenglong CHEN Dexin WANG Xiaoqiang |
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| Affiliation: | 1. Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao 266000, China;2. Shanxi Tobacco Company, Xi'an 712000, China;3. Guizhou Tobacco Company, Guiyang 550004, China;4. Shangluo Tobacco Company of Shaanxi Province, Shangluo, Shannxi 726000, China;5. Linyi Tobacco Company of Shandong Province, Linyi, Shandong 276002, China;6. Hainan Tobacco Company, Haikou 571199, China |
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| Abstract: | In order to clarify the biocontrol characteristics of Bacillus velezensis GY1, Bacillus amyloliquefaciens GY10, and Bacillus subtilis GY12, antibacterial spectrum, secondary metabolites, volatile substances, rhizosphere colonization ability and growth-promoting ability of the strains were studied by compatible test, double petri dish assay and pot test. The results showed that GY1, GY10, and GY12 can significantly promote seed germination and growth of tobacco. The three strains have a broad antimicrobial spectrum and have significant inhibitory effects against Alternaria alternata, Phytophthora parasitica var. nicotianae and Fusarium spp. In addition, all of the tree strains have the ability to produce siderophore and protease. The fermentation supernatants and volatile substances of the three strains also have a significant inhibitory effect against A. alternata and P. parasitica var. Nicotianae. The secreted proteins of the strains have good thermal stability and significant in vitro antibacterial activity on A. alternata, and have high tolerance to acid environments. The results of rhizosphere colonization assay indicated that GY1-GY10-GY12 compounding could increase the colonization of each biocontrol bacteria in the tobacco rhizosphere compared with a single strain. The resuls from this study provide guidance for in-depth exploration of the biocontrol characteristics of strains GY1, GY10, and GY12 and the industrial development of biocontrol strains. |
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| Keywords: | Bacillus spp. Phytophthora parasitica Alternaria alternata biocontrol mechanism rhizosphere colonization |
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