山东烟区首次发现番茄斑萎病毒侵染

为确定番茄斑萎病毒（TSWV）在山东烟草上的侵染，基于GenBank中已有的TSWV基因组序列设计该病毒特异性引物，通过总RNA提取，RT-PCR扩增，全基因组序列测定和拼接分析等，结果表明，山东临沂疑似病样中含有TSWV，该TSWV分离物具有3条RNA链，大小分别为8911、4773和2971 bp，与国内已报道的其他TSWV分离物核酸序列同源性均在99.0%以上，蛋白氨基酸序列同源性均在98.0%以上。系统进化分析结果显示，该分离物与已报道的TSWV云南分离物聚类到同一分支中，推测山东烟区中的TSWV可能由云南烟区通过带毒介体的迁入而传入。该研究为山东烟区TSWV的发生流行溯源和该危险性病毒病的精准测报及防控提供科学依据。

The Infection of TSWV was First Found in Shandong Tobacco Growing Areas

In order to determine the infection of TSWV in Shandong tobacco, specific primers were designed based on the existing TSWV genome sequence in GenBank. Through total RNA extraction, RT-PCR amplification, whole genome sequence determination and splicing analysis, the results showed that the suspected samples of Linyi, Shandong province were infected by TSWV. In order to further determine the whole genome sequence of the TSWV Linyi isolates, TSWV whole genome amplification primers were designed and synthesized. The results of cloning, sequencing and splicing analysis showed that the three RNA chains segments of the Shandong isolate are 8911, 4773 and 2971 bp in length, with more than 99.0% homology of nucleic acid sequence and 98.0% homology of protein sequence with the other reported Chinese isolates. Phylogenetic analysis showed that the Shandong isolate and the reported Yunnan TSWV isolate were clustered together in the same branch. It is speculated that TSWV in Shandong tobacco growing areas may have been introduced from Yunnan tobacco areas through the migration of toxic mediators. This study provides scientific evidence for the traceability of TSWV epidemics in Shandong tobacco growing areas and the accurate prediction and prevention of this dangerous viral disease.