不同品种彩粒小麦苗麦绿素提取工艺优化及其含量比较

以类黄酮、蛋白质含量和超氧化物歧化酶(Superoxide Dismutase,SOD)活性作为评价指标,试验采用水浸提法,通过单因素试验和L9(34)正交试验,考察了浸提温度、浸提时间和料液比对山农紫小麦、黑小麦031244、紫小麦204W17、黑小麦202W20 4个品种彩粒小麦苗麦绿素制备的影响,确定其提取的最佳工艺,并对其麦绿素含量进行比较。结果表明,紫小麦204W17苗麦绿素中类黄酮和蛋白质含量均最高,分别为40.21mg/100g和49.04%,其提取的最佳工艺条件为浸提温度25℃,浸提时间2.0h,浸提料液比1∶4;山农紫小麦苗麦绿素中SOD含量最高,为1865.3U/100g,其提取的最佳工艺条件为浸提温度30℃,浸提时间2.5h,浸提料液比1∶3。综合评价得出紫小麦204W17苗和山农紫小麦苗的麦绿素含量更高。

Extraction Technology Optimization and Content Comparison of Barley Green from Different Varieties of Wheat

The single factor experiment and L9(34)orthogonal experiment are conducted to investigate the effect of extracting temperature,extracting time and the ratio of solid to liquid on the preparation of barley green from four varieties of color-grained wheat seedlings including Shannong purple wheat,triticale 031244,purple wheat 204 W17 and triticale 202 W20 according to the content of flavonoids,protein and superoxide dismutase(SOD)by water extraction method,to determine the optimum extraction process of barley green and to compare its content among these varieties.The results show that the content of flavonoids and protein in barley green from purple wheat 204 W17 seedlings is the the highest of 40.21 mg/100 g and 49.04% respectively,and the optimal extracting conditions are concluded as follows:the extracting temperature is 25℃,the extracting time is 2.0 hand the ratio of solid to liquid is 1∶4.The content of SOD in barley green from Shannong purple wheat seedlings is the highest of 1865.3 U/100 g,and its optimal extracting conditions are concluded as follows:the extracting temperature is 30 ℃,the extracting time is 2.5 hand the solid-liquid ratio is 1∶3.Comprehensive evaluation shows that both purple wheat 204 W17 and Shannong purple wheat seedlings have higher-content barley green.