Composition and biosynthesis of sterols in selected marine phytoplankton |
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| Authors: | Don S Lin Ajmal M Ilias William E Connor Richard S Caldwell H Therese Cory G Doyle Daves Jr |
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| Affiliation: | (1) Section of Clinical Nutrition-Lipid Metabolism, Department of Medicine, University of Oregon Health Sciences University, 97201 Portland, OR;(2) Department of Fisheries and Wildlife, Oregon State University, Marine Science Center, 97365 Newport, OR;(3) Department of Chemistry and Biochemical Sciences, Oregon Graduate Center, 97006 Beaverton, OR;(4) Present address: Marine Research Laboratory, Northwestern Aquatic Sciences, Inc., PO Box 1437, 97365 Newport, OR |
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| Abstract: | Six species of phytoplankton,Pseudoisochrysis paradoxa, Isochrysis galbana, Monochrysis lutheri, Platymonas suecica, Thalassiosira fluviatilis and aChaetoceros species, were cultured in the laboratory and their sterol contents analyzed utilizing digitonin precipitation, thin layer
and gas chromatography and gas chromatography-mass spectrometry. A total of 7 sterols were found in phytoplankton. The occurrence
of these sterols, cholest-5-en-3β-ol, cholest-5,22-dien-3β-ol, 24-methylcholesta-5,24(28)-dien-3β-ol, 24-methylcholest-5-en-3β-ol,
24-methylcholesta-5,22-dien-3β-ol, 24-ethylcholest-5-en-3β-ol and 24-ethylcholest-5,22-dien-3β-ol, differed significantly
among the various phytoplankton species. Cultures ofP. paradoxa biosynthesized both of the sterols found in this species when incubated in the presence of14C- or3H-mevalonic acid for 0.5–9 days. These sterols were cholesterol and 24-methylcholesta-5,22-dien-3β-ol. Since 5 of the sterols
found in the phytoplankton commonly occur in mollusks which feed on phytoplankton, it is likely that at least some of the
tissue sterols in mollusks are of dietary origin.
Research trainee, HL 07295-02, National Heart, Lung and Blood Insitute. |
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