麦麸多肽的分离纯化及抗氧化功能研究

采用碱性蛋白酶Alcalase 水解麦麸蛋白制备麦麸多肽，利用SephadexG-25 和DEAE-32 对麦麸多肽进行纯化，醋酸纤维素薄膜电泳法测定麦麸多肽纯度，用硝基四氮唑蓝(NBT)还原实验、脱氧核糖法检测其对超氧阴离子自由基(O2·)和羟自由基(·OH)的清除能力，分光光度法测定小鼠红细胞溶血和肝线粒体膨胀程度。结果表明，分离纯化的麦麸多肽对O2·和·OH 的清除率别为53.16%、62.39%，并且具有明显抑制氧化溶血现象和·OH 诱导线粒体肿胀的作用，说明麦麸多肽具有较强的抗氧化功能。

Study on Purification and Antioxidation in vitro of Wheat Bran Peptide

Wheat bran peptides were prepared by alkaline protease (Alcalase) hydrolysis and then purified with Sephadex G- 25 and DEAE-32 column chromatography. The cellulose acetate electrophoresis was used to determine the purity of wheat bran peptide with antioxidant activity. The surperoxide anion radical and hydroxyl radical scavenging activities of wheat bran peptide were detected through nitroblue tetrazolium (NBT) test and deoxyribose method. The erythrocatalysis extent and swelling extent of liver mitochondria were detected using spectrophotometric method. The results showed that the surperoxide anion radical and hydroxyl radical scavenging activities of purified wheat bran peptide are 53.16% and 62.39%, respectively, and the purified wheat bran antioxidant peptide can inhibit swelling of liver mitochondria and hemolysis of red blood cells, indicating that it has significant antioxidation.