小球藻中海藻油的提取工艺研究

本实验以小球藻为原料，利用反复冻融技术破碎细胞壁，并用乙醇作提取剂提取海藻油。研究了乙醇浓度、液料比、冻融次数以及提取温度等因素对油脂提取率的影响。实验结果表明，最佳工艺条件为反复冻融1次、液料比3:1(V/W)、乙醇浓度95%、提取温度45℃，出油率达24.28%。小球藻经反复冻融提取海藻油，提取较完全，适宜大量生产，有着较广阔的应用前景。与藻泥直接提取及冷冻干燥后提取相比，提取效率高，且差异极显著。

Study on Extraction Technology of Algae Oil from Chlorella

The repeated freezing and thawing technique was taken to break cell wall of chlorella and then the algae oil was extracted with ethanol. The effects of ethanol concentration, ratio of material to solvent, times of freezing and thawing, and extraction temperature on the oil yield were investigated. The results showed that the optimum ethanol concentration, ratio of solvent to material, times of freezing and thawing, and extraction temperature are 95%, 3:1 (V/W), once, and 45 ℃, respectively. Under these conditions, the algae oil yield reaches 24.28%. Compared to traditional methods, the combined method of repeated freezing-thawing and ethanol extraction can completely extract the algae oil with a high yield, and is suitable for large-scale industrial production.