SYBR Green实时荧光定量PCR检测大豆转基因成分

采用SYBR Green实时荧光定量PCR技术，建立了食品中大豆转基因成分的定量检测方法。通过设计特异引物，扩增内源参照基因lectin和转基因靶基因CP4EPSPS，建立两种基因的拷贝数-CT标准曲线，根据标准曲线方程计算样品中的转基因含量，并且通过熔解曲线分析扩增反应特异性。结果表明，lectin和CP4EPSPS基因标准曲线线性关系好，R2值分别为0.9984和0.9953，方法的回收率为95%～110%，检测限为0.01%。本检测方法具有快速、灵敏、准确、特异、高通量等优点，可以作为食品中大豆转基因成分的定量检测方法。

Detection of Transgenic Components in Soybean Products by SYBR Green Real-Time PCR

A quantitative method to detect the transgenic component in soybean by using real-time PCR technique based on fluorescence dye SYBR Green was investigated in this study. The endogenous lectin gene and transgenic target CP4EPSPS gene were amplified through the design of specific primers, and the transgenic content was then calculated according to the standard curve equation. Meanwhile the specificity of PCR amplification was analyzed with corresponding melting curves .The results showed that the standard curves of lectin and CP4EPSPS genes have good linear relationship, and their R2 values are 0.9984 and 0.9953, respectively. Moreover, the recovery rate of the method is between 95% and 110% and the detection limit is 0.01%. In conclusion, this method is fast, sensitive, simple, accurate, specific and of high throughput, and can be used to quantificationally detect transgenic components in soybean products.