高效液相色谱柱前衍生法测定蜜饯中的甜蜜素

目的：建立简便、准确、灵敏的测定蜜饯中甜蜜素含量方法。方法：在酸性介质条件下，溶液中的甜蜜素同次氯酸钠溶液发生反应，其衍生产物在紫外-HPLC 法下测定。结果：方法最低检出限为0.05g/kg，其在0.02～1.00mg/ml 范围内呈线性关系，相关系数为0.9995，方法的相对标准偏差为1.56%～4.49%，回收率为91.5%～98.5%，检测结果与GB/T5009.97 - 2003 气相色谱法无显著差异。结论：本法灵敏度高，操作简单，稳定性和重复性好，适合于蜜饯中甜蜜素含量的测定。

Determination of Cyclamate in Preserved Fruits by High-performance Liquid Chromatography Coupled with Pre-column Derivatization

Objective: to establish a simple, accurate and sensitive method for determination of cyclamate in preserved fruits by high-performance liquid chromatography with pre-column derivatization. Method: Through the reaction between cyclamate and sodium hypochlorite under acid medium, N,N-dichloro- cyclohexylamine was determined by UV-HPLC. Results: The detection limit is 0.05 g/kg. The calibration curve is linear in the range of 0.02 to 1.00 mg/ml with a correlation coefficient of 0.9995. The relative standard deviation is 1.56% to 4.49% and the recovery rate is in the range of 91.5% to 98.5%. The determination result has no significant deference from that by the method of GB5009.97 - 2003. Conclusion: The method is sensitivity, simple and stable and has good repetition. It can be used for the determination of cyclamate in preserved fruit.