改良FTA-PCR快速检测单核细胞增生李斯特氏菌研究

目的：建立快速、特异、灵敏的单核细胞增生性李斯特氏菌的PCR 检测方法。方法：以单核细胞增生李斯特氏菌的hlyA 基因作为靶序列设计一对引物，运用改良的FTA-PCR 法对单核细胞增生李斯特氏菌进行鉴定和检测。结果：扩增产物为136bp 的片断，此方法较原有方法灵敏度增强，可达到1.1 × 102CFU/ml。结论：改良的FTA 卡法特异性强、灵敏度高，有效缩短了检验周期，从传统的7～14d 缩短到1～1.5d。

Improved Flinders Technology Associates (FTA) Filter Paper-based DNA Extraction with Polymerase Chain eaction (PCR) for Rapid Detection of Listeria monocytogenes

Objective: To set up a detection approach of Listeria monocytogenes based on PCR. Methods: A pair of primers ere designed with hlyA gene of Listeria monocytogenes as target sequence for its detection by improved FTA-PCR method. esults: A 136-bp DNA fragment was amplified from the primers. The sensitivity of the improved FTA-PCR method was up o 1.1 × 102 CFU/ml. Conclusion: This method is rapid, sensitive and accurate, with high specificity, which can shorten the etection time from 7 to 14 days to 1 to 1.5 days.