| 液相色谱-串联质谱法测定肉肠中莱克多巴胺残留量的研究 |
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| 引用本文： | 张丽芳,王霄旸,张 涛,裘敏琪,薛飞群.液相色谱-串联质谱法测定肉肠中莱克多巴胺残留量的研究[J].食品科学,2009,30(8):197-200. |
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| 作者姓名： | 张丽芳 王霄旸 张 涛 裘敏琪 薛飞群 |
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| 作者单位： | 中国农业科学院上海兽医研究所兽药安全评价与残留研究重点开放实验室，农业部动物寄生虫学重点开放实验室 |
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| 摘 要： | 建立了肉肠中莱克多巴胺残留量固相萃取-液相色谱-串联质谱分析方法。肉肠样品经甲醇提取，提取物经葡萄糖醛酸酶酶解后，固相萃取小柱净化。选用多反应监测模式进行测定。通过基质效应的考察，确定了定量校正的方法。结果显示，莱克多巴胺添加浓度在0.5～4μg/kg范围内时，其峰面积和浓度呈良好的线性范围，相关系数R2大于0.99。本方法最低检测限为0.15μg/kg。
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| 关 键 词： | 莱克多巴胺 固相萃取 液相色谱-串联质谱 残留 |
| 收稿时间： | 2008-05-16 |
Determination of Ractopamine Residues in Sausage by High Performance Liquid Chromatography-Tandem Mass Spectrometry |
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| ZHANG Li-fang WANG Xiao-yang ZHANG Tao QIU Min-qi XUE Fei-qun.Determination of Ractopamine Residues in Sausage by High Performance Liquid Chromatography-Tandem Mass Spectrometry[J].Food Science,2009,30(8):197-200. |
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| Authors: | ZHANG Li-fang WANG Xiao-yang ZHANG Tao QIU Min-qi XUE Fei-qun |
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| Affiliation: | (Key Open Laboratory of Veterinary Drug Safety Evaluation and Residues Research, Shanghai Veterinary Research Institute, Chinese
Academy of Agricultural Sciences, Key Laboratory of Animal Parasitology, Ministry of Agriculture, Shanghai 200232, China) |
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| Abstract: | A liquid chromatography-tandem mass spectrometry (LC-MS/MS) coupled with solid phase extraction method was developed for determining the residual ractopamine in sausage. Sausage samples were extracted with methanol, hydrolyzed with β-glucuronidase, purified by solid phase and detected by the multiple reaction monitoring mode. After investigation of matrix effect, the quantitative correction method was confirmed. The results showed that the peak area and concentration of ractopamine present a good linear relationship in the concentration range of 0.5 to 4μg/kg (R2 > 0.99), and the detection limit is 0.15 μg/kg. |
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| Keywords: | ractopamine solid phase extraction LC-MS/MS residues |
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