虾夷扇贝脏器硫酸酯多糖的制备及性质研究

采用氯磺酸- 甲酰胺法对扇贝脏器粗多糖进行硫酸化修饰。经红外光谱分析表明，修饰产物具有典型的多糖吸收峰和硫酸基吸收峰。硫酸酯多糖经Bio-Gel P-6 凝胶过滤柱分离后获得SSVP-I 和SSVP-II 两种多糖组分，其中组分SSVP-I 的平均分子量约为5900u，硫酸基取代度为0.98，主要由鼠李糖、岩藻糖、阿拉伯糖、木糖、甘露糖、半乳糖、葡萄糖以摩尔比为1.0:1.3:2.4:3.0:1.3:4.1:2.5 组成。体外抗凝血实验表明，SSVP-I 能显著延长人活化部分凝血活酶时间(APTT)、凝血酶原时间(PT)和凝血酶时间(TT)，且具有一定的剂量- 效应依赖关系。

Study on Preparation and Physicochemical Analysis of Sulphated Scallop (Patinopecten yessoensis) Viscus Polysaccharides

The crude scallop (Patinopecten yessoensis) viscus polysaccharides (SVP) were sulphated with chlorosulfonic acidformamide method, and typical absorption peaks of polysaccharide and sulfate were shown in IR spectra of the sulfated SVP (SSVP). Two polysaccharide compositions SSVP-I and SSVP-II were obtained from the sulfated polysaccharide separated by gel-filtration chromatography with Bio-Gel P-6, and the physicochemical characteristics of SSVP-I was investigated. The results showed that the average molecular weight of SSVP-I is 5900 u, its degree of sulphate group substitution is 0.98, and its monosaccharide composition is Rha, Fuc, Ala, Xyl, Man, Gal and Glu in the molar ratio of 1.0:1.3:2.4:3.0:1.3:4.1:2.5. The anticoagulation experiment in vitro indicated that SSVP-I can prolong activated partial thromboplastin time (APTT), prothrombin time (PT) and thrombin time (TT) of human with a certain dose-effect relationship.