气相色谱外标法测定环十五内酯

进行蒜头果油合成环十五内酯的定量分析方法研究，用气相色谱外标法建立环十五内酯的定量分析方法，以环十五内醋的峰面积(A)对应的浓度(C)进行线性回归分析，得到通过原点的回归方程为：A=2238.7C(r=0.9993)，可以利用单点校正对标准曲线进行校正，回收率为95.11%～103.3%，并利用该法跟踪测定了蒜头果油合成环十五内酯产物的含量。

Determination of Cyclopentadecanolide Synthesized from Malania oleifera Chun et S.K. Lee Kernel Oil by Gas Chromatography with External Standard Method

Gas chromatography (GC) with area-external standard method was applied to quantitative analysis of cyclopentadecanolide in its synthesis from Malania oleifera Chun et S.K. Lee kernel oil. A linear regression equation was obtained, which was A = 2238.7C (r = 0.9993), where A was the GC peak area, and C was the cyclopentadecanolide concentration, and the standard curve was validated by single-point external bracketing standard. The recoveries of cyclopentadecanolide in 3.578 mg of sample at the spiked amounts of 1.977, 3.954 and 5.931 mg were 95.11% to 103.3%, the RSDs were 1.51% to 2.19% (n = 3). The determination results of cyclopentadecanolide content in four prepared sample solutions were 2.14 to 4.52 mg/ml, and the RSDs were 0.66% to 2.36% (n = 3).