水产品中恩诺沙星残留的一步法酶联免疫检测研究

采用过碘酸钠氧化法合成了辣根过氧化物酶(HRP)标记的恩诺沙星抗体，建立一步式直接竞争酶联免疫吸附检测(ELISA)技术，并以鳗鱼为样本进行了恩诺沙星残留的加标检测。结果表明，所制备的酶标记抗体效价达到10000 以上，与同族其他药物及族外药物没有显著的交叉反应；所建立的一步式ELISA 法检测限约为10μg/kg。在10～40μg/kg 浓度范围内，加标鳗鱼样本中恩诺沙星的检测回收率在70% 以上，相对平均偏差小于6%，检测时间缩短到2h 以内，约为传统两步式ELISA 法的一半左右。

Development of One-step Enzyme-linked Immunoassay for Determining Enrofloxacin Residues in Sea Foods

In this study, horseradish peroxidase (HRP) was used to label anti-enrofloxacin antibody through a sodium periodate oxidation method. A one-step competitive direct enzyme-linked immunosorbent assay (ELISA) was developed for determining enrofloxacin in sea foods. The results showed that the titer of labeled antibody was was higher than 10000, and no significant cross-reactivity was observed with other fluoroquinolones and other groups of antibiotics. The detection limit was 10 μg/kg. For eel samples spiked with enrofloxacin from 10 to 40 μg/kg, the average recoveries were above 70% and the relative standard deviations were lower than 6%. The determination time by this method was shortened to less than 2 h due to pre-coating and blocking in microplates, which was about half of that by traditional two-step ELISA.