LAL/GNB法筛选辐照鸡肉的应用研究

目的：探讨内毒素检测(LAL)与革兰氏阴性菌计数(GNB)相结合的方法可否应用于辐照鸡肉的筛选。方法：市售鸡肉丁以50g 每份分装后，以γ射线进行辐照处理，剂量分别为0、2、6kGy，每个剂量各12 份，通过革兰氏阴性菌的培养计数估计样品中存在的活的革兰氏阴性菌的量，通过内毒素浓度定量估计活的和死亡的革兰氏阴性菌总量，比较两者差异来判定是否经过辐照处理。结果：辐照剂量为6kGy 时，12 个样品被检测出经过辐照处理。辐照剂量为2kGy 时，8 个样品被检测出经过辐照处理，4 个样品检测出未经过辐照处理。辐照剂量为0kGy 时，12 个样品均被检测出未经过辐照处理。结论：LAL/GNB 法可以应用于辐照鸡肉的筛选。

Limulus Amoebocyte Lysate (LAL) Assay Coupled with Gram Negative Bacteria (GNB) Count for Detecting γ-Ray-irradiated Chicken

Objective: To explore the availability of LAL/GNB method for screening irradiated chicken. Methods: Every 50 g of saled diced chicken was packaged into a sample and then every 12 chicken samples were irradiated byγ-ray of 0, 2 and 6 kGy, respectively. The viable GNB number in chicken sample was estimated by GNB count method, and the total number of GNB was estimated according to bacterial endotoxin concentration. The difference in the two numebers was used to judge if chicken sample had been irradiated or not. Results: In the 6 kGy-dose group, all 12 samples were detected to be irradiated. In the 4 kGydose group, 8 samples were detected to be irradiated, except 4 samples. In the control group, all 12 samples were detected to be not irradiated. Conclusion: The LAL/GNB method is available for detecting irradiated chicken.