| 基于ITS序列分析对疑似白羊肚菌株的分子鉴定 |
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| 引用本文： | 侯 军,林晓民,江 芸,牛洪江,刘 峰,王常容,刘 方. 基于ITS序列分析对疑似白羊肚菌株的分子鉴定[J]. 食品科学, 2009, 30(5): 141-144. DOI: 10.7506/spkx1002-6630-200905032 |
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| 作者姓名： | 侯 军 林晓民 江 芸 牛洪江 刘 峰 王常容 刘 方 |
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| 作者单位： | 1.河南科技大学林学院 2.南开大学微生物系 |
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| 基金项目： | “十一五”国家科技支撑计划重点项目(2006BAD27B06) |
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| 摘 要： | 通过提取疑似白羊肚菌株基因组DNA，采用通用引物ITS1和ITS4扩增ITS序列，利用GenBank中的BLAST软件搜索同源序列进行比对，并构建系统发育树，对疑似菌株白羊肚进行了初步分子鉴定。结果表明，在GenBank数据库中登录的有26 个菌株与白羊肚ITS 序列相似度为99%，其中Pleurotus eryngii (杏鲍菇)15 个，P. nebrodensis(白灵菇)11 个。根据rDNA ITS 区序列分析准则和白羊肚菌褶呈网格状，菌盖为白色的形态特征，确定疑似白羊肚菌株为与白灵菇(P. nebrodensis)和杏鲍菇(P. eryngii)高度近缘的一个菌株。
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| 关 键 词： | 白羊肚 ITS 白灵菇 杏鲍菇 亚种 分子鉴定 |
| 收稿时间： | 2008-03-28 |
Molecular Identification of Suspected “Baiyangdu” Strain Based on Internal Transcribed Spacer Sequence Analysis |
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| HOU Jun,LIN Xiao-min,JIANG Yun,NIU Hong-jiang,LIU Feng,WANG Chang-rong,LIU Fan. Molecular Identification of Suspected “Baiyangdu” Strain Based on Internal Transcribed Spacer Sequence Analysis[J]. Food Science, 2009, 30(5): 141-144. DOI: 10.7506/spkx1002-6630-200905032 |
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| Authors: | HOU Jun LIN Xiao-min JIANG Yun NIU Hong-jiang LIU Feng WANG Chang-rong LIU Fan |
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| Affiliation: | (1.College of Forestry, Henan University of Science Technology, Luoyang 471003, China；2.Department of Microbiology, Nankai University,Tianjin 300071, China ) |
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| Abstract: | Based on the amplification of the internal transcribed spacer (ITS) sequence with the primers of ITS1 and ITS4, the suspected “Baiyangdu” strain was identified. The results showed that the ITS sequence of 26 strains (15 P. eryngii and 11 P. nebrodensis) registered in GenBank has similarity of 99% to that of“ Baiyangdu” strain. According to the criterion of ITS sequence analysis and characteristics of white pileus and reticulate lamella, the“ Baiyangdu” is identified as a highly affinis strain of P. nebrodensis and P. eryngii. |
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| Keywords: | “Baiyangdu”strain ITS P.nebrodensis P.eryngii molecular identification |
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