| 刺梨中芦丁成分的定量测定 |
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| 引用本文： | 田宏哲,周艳明,赵瑛博,臧 建,胡 睿. 刺梨中芦丁成分的定量测定[J]. 食品科学, 2009, 30(10): 203-205. DOI: 10.7506/spkx1002-6630-200910045 |
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| 作者姓名： | 田宏哲 周艳明 赵瑛博 臧 建 胡 睿 |
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| 作者单位： | 沈阳农业大学分析测试中心 |
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| 摘 要： | 建立了测定刺梨果实中芦丁含量的高效液相色谱方法。刺梨粉用乙醇超声提取，提取液过滤后通过旋转蒸发仪浓缩，浓缩液离心后取上清液，过0.45μm 滤膜后用流动相稀释，进行反相色谱测定。本方法线性范围为0.1～200 μg/ml，线性相关系数R2 = 0.9988。
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| 关 键 词： | 刺梨 芦丁 定量分析 |
| 收稿时间： | 2008-07-18 |
Quantitative Analysis of Rutin in Rosa roxburghii Tratt Fruit by HPLC |
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| TIAN Hong-zhe,ZHOU Yan-ming,ZHAO Ying-bo,ZANG Jian,HU Rui. Quantitative Analysis of Rutin in Rosa roxburghii Tratt Fruit by HPLC[J]. Food Science, 2009, 30(10): 203-205. DOI: 10.7506/spkx1002-6630-200910045 |
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| Authors: | TIAN Hong-zhe ZHOU Yan-ming ZHAO Ying-bo ZANG Jian HU Rui |
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| Affiliation: | (Analyzes and Testing Center, Shenyang Agricultural University, Shenyang 110161, China) |
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| Abstract: | A method for determining rutin in Rosa roxburghii Tratt fruit by high performance liquid chromatography (HPLC) was developed. The powder of Rosa roxburghii Tratt fruit was extracted with ethanol under the assistance of ultrasonic, and the rutin extract was filtrated, concentrated by a rotary evaporator and centrifuged. The supernatant was filtered through a 0.45 μm filter membrane, diluted with mobile phase, and analyzed by reversed-phase liquid chromatography. The linear range of this method was 0.1 to 200 μg/ml, and the determination coefficient was 0.9988. The precision RSD was 2.10% (n = 5). The average spike recovery of rutin was 94.21%, and the RSD was 2.34%. |
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| Keywords: | Rosa roxburghii Tratt rutin quantitative analysis |
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