鸡肉组织中氯羟吡啶残留超高效液相色谱检测方法研究

对鸡肉组织中氯羟吡啶残留高效液相色谱方法进行了研究，建立了鸡肉组织中氯羟吡啶残留超高效液相色谱检测方法。取鸡肉样品，用乙腈提取，正己烷脱脂，过氧化铝B 柱，用20ml 甲醇洗脱，减压蒸干，残留物用甲醇溶解，紫外检测器在267nm 波长下测定，流动相为乙腈:水(10:90，V/V)。氯羟吡啶浓度在10～1000μg/L 范围内，呈良好的线性，相关系数＞ 0.999，在10、50、100μg/kg 三个添加水平，平均回收率60.5%～94.6%，日内变异系数在4.2%～10.2%之间，日间变异系数在6.6%～12.7%之间，方法检测限为5μg/kg，定量限为15μg/kg。

Determination of Clopidol Residue in Chicken by Ultra Performance Liquid Chromatography

An ultra performance liquid chromatography (UPLC) method for determination of clopidol in chicken was developed. The clopidol residue in chicken was extracted by acetonitrile, defatted by n-hexane, purified by alumina B cartridges, analyzed by HPLC with UV detector at 267 nm and acetonitrile-water (10:90, V/V) solvent as mobile phase, and quantified by using external standard calibration curve. The results showed that there was a nice linear relationship (r ＞ 0.999) when clopidol concentration was in the range of 10 to 1000 μg/L. The average recoveries at three addition levels (10, 50 and 100 μg/kg) were 60.5% to 94.6%. The within-day and inter-day variation coefficients were between 4.2% and 10.2% and between 6.6% and 12.7%, respectively. The limit of detection was 5 μg/kg, and the limit of quantification was 15 μg/kg.