赭曲霉毒素A单克隆抗体免疫亲和柱的制备

制备了赭曲霉毒素A(OTA)单克隆抗体免疫亲和柱，并用间接竞争ELISA和HPLC法评价了免疫亲和柱的性能，其柱容量(结合OTA的能力)约为200ng，加标回收率为90.38%～100.1%，可反复使用3次。 建立了免疫亲和柱-HPLC联用分析谷物中OTA的方法，最低检出限为0.2μg/kg，线性范围为0.6～400μg/kg，OTA加标量为1～10μg/kg时谷物样品中的回收率为78.7%～87.1%，变异系数小于6.5%。用此法检测了大米、小麦、玉米和玉米饲料等15份市售样品，检出率为46.7%，其中OTA的最高含量为0.785μg/kg。

Development of Immunoaffinity Column of Anti-ochratoxin A Monoclonal Antibody

An immunoaffinity column (IAC) was prepared with anti-ochratoxin A (OTA) monoclonal antibody and the efficiency of IAC was analyzed by indirect competitive enzyme-linked immunosobent assay (IC-ELISA) and high performance liquid chromatography (HPLC). Results indicated that this IAC had a high column capacity of binding OTA (200 ng) and the recovery ranging from 90.38 % to 100.1 %, and could be used repeatedly 3 times. IAC linked with HPLC was used to detect OTA contents in 15 cereal samples. The limit of detection (LOD) of OTA by this method was 0.2 μg/kg. Samples with OTA levels from 0.6 to 400 μg/kg could be quantitified by this method. The recovery of spiked OTA at levels of 1 to 10 μg/kg ranged from 78.7 % to 87.1 % with coefficients of variation (CVs) less than 6.5 %. This method was applied to detect 15 saled cereal samples. The positive detection rate was 46.7 %, and the highest OTA content detected was 0.785 μg/kg.