| 青海藏系绵羊肉中肌苷酸、肌苷的含量测定 |
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| 引用本文： | 刘文惠,星玉秀,董 琦,皮 立,胡凤祖. 青海藏系绵羊肉中肌苷酸、肌苷的含量测定[J]. 食品科学, 2009, 30(8): 238-240. DOI: 10.7506/spkx1002-6630-200908053 |
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| 作者姓名： | 刘文惠 星玉秀 董 琦 皮 立 胡凤祖 |
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| 作者单位： | 1.中国科学院西北高原生物研究所 2.中国科学院研究生院 |
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| 摘 要： | 采用高效液相色谱法对藏系绵羊肉中的肌苷、肌苷酸进行定量分析。色谱柱：Kromasil C18 柱(4.6mm× 250mm，5μm)；流动相：甲醇-水(95:5，V/V)；流速1ml/min；紫外检测波长：254nm。结果表明：肌苷酸、肌苷分别在12.96～207.50mg/L和1.40～22.42mg/L范围内呈线性关系，回收率分别为97.98%、98.26%，该方法可用于动物肌肉组织中的肌苷酸、肌苷含量的测定。
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| 关 键 词： | 高效液相色谱 肌苷酸 肌苷 藏系绵羊肉 |
| 收稿时间： | 2008-06-24 |
Quantitative determination of inosinic acid and inosine in Tibetan sheep Muscle by HPLC |
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| LIU Wen-hui,XING Yu-xiu,DONG Qi PI Li HU Feng-zu. Quantitative determination of inosinic acid and inosine in Tibetan sheep Muscle by HPLC[J]. Food Science, 2009, 30(8): 238-240. DOI: 10.7506/spkx1002-6630-200908053 |
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| Authors: | LIU Wen-hui XING Yu-xiu DONG Qi PI Li HU Feng-zu |
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| Affiliation: | (1. Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810008, China2. Graduate University of Chinese Academy of Sciences, Beijing 100049, China |
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| Abstract: | The quantitative determination of inosinic acid and inosine was conducted by high performance liquid chromatography(HPLC)．The separation was carried out with analytical Kromasil C18 column (5 μm, 4.6 mm×250 mm), the mobile phase consisted of methanol and water (95:5, V/V), the flow rate was 1 ml/min and UV detection was performed at 254 nm. The results showed that there are good linear relationships between inosinic acid concentration and its HPLC peak area and between inosine concentration and its HPLC peak area in the range of 12.96 to 207.50 mg/L and 1.40 to 22.42 mg/ L, respectively. The average recovery rates were 97.98% and 98.26% for inosinic acid and inosine respectively．This method is fit for the quantitative analysis of inosinic acid and inosine in muscle of animals. |
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| Keywords: | HPLC inosinic acid inosine Tibetan sheep muscle |
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