| 实时荧光PCR技术定量检测转基因豆粉的研究 |
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| 引用本文： | 白卫滨,孙建霞,程国灵,罗云波,姜桂传,黄亚东. 实时荧光PCR技术定量检测转基因豆粉的研究[J]. 食品科学, 2009, 30(6): 238-242. DOI: 10.7506/spkx1002-6630-200906056 |
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| 作者姓名： | 白卫滨 孙建霞 程国灵 罗云波 姜桂传 黄亚东 |
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| 作者单位： | 1.中国农业大学食品科学与营养工程学院 2.山东省农业管理干部学院 3. 暨南大学生物医药开发中心 |
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| 基金项目： | 山东省教育厅资助项目(J06I55) |
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| 摘 要： | 本实验以美国、阿根廷、巴西豆粉、中国豆粉和转基因大豆粉标准品为材料，利用设计的特异性引物和探针，建立了实时荧光定量PCR 技术检测抗草甘膦转基因豆粉的方法，成功检测出美国、阿根廷、巴西抗草甘膦转基因豆粉和中国豆粉的转基因含量分别为5.37%、3.96%、2.26% 和0，确定了荧光PCR 技术检测抗草甘膦转基因豆粉的灵敏度为0.001%，稳定性良好。
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| 关 键 词： | 转基因豆粉 实时荧光PCR 检测 灵敏度 稳定性 |
| 收稿时间： | 2008-05-09 |
Study on Quantitative Detection of Glyphosate-resistant Genetically Modified Soybean Powder by Real-time Fluorescence PCR Method |
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| BAI Wei-bin,SUN Jian-xia,TRINH Quoc-linh,LUO Yun-bo,JIANG Gui-chuan,HUANG Ya-dong. Study on Quantitative Detection of Glyphosate-resistant Genetically Modified Soybean Powder by Real-time Fluorescence PCR Method[J]. Food Science, 2009, 30(6): 238-242. DOI: 10.7506/spkx1002-6630-200906056 |
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| Authors: | BAI Wei-bin SUN Jian-xia TRINH Quoc-linh LUO Yun-bo JIANG Gui-chuan HUANG Ya-dong |
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| Affiliation: | (1. College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China；2. Shandong Agricultural Administrators College, Jinan 250100, China；3. Biopharmaceutic R and D Center of Jinan University, Guangzhou 510630, China) |
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| Abstract: | A real-time fluorescence PCR method was developed for quantitative detection glyphosate-resistant genetically modified soybean powder. The detection results showed that the genetically modified (GM) contents of soybean powder from USA, Argentina, Brazil and China are 5.37%, 3.96%, 2.26% and 0, respectively. The sensitivity of established detection method is 0.001% and its stability is fairly good. |
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| Keywords: | genetically modified soybean powder real-time fluorescence PCR detection sensitivity stability |
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