高效液相色谱- 电喷雾三级四极杆质谱测定苹果、山楂、番茄制品中棒曲霉素的研究

建立了苹果、山楂、番茄制品中棒曲霉素总量的提取方法和液相色谱串联质谱确证方法。清汁样品用乙腈稀释后直接过MycoSep 228 真菌霉素多功能净化柱；浊汁、固体样品用果胶酶水解后，再用乙酸乙酯提取样品中棒曲霉素，浓缩后经MycoSep 228 柱净化，用Atlantis dC18 色谱柱分离，液相色谱串联质谱测定，电喷雾负离子(ESI －)多反应模式下监测；实验结果表明，样品中棒曲霉素浓度在1.0～200μg/kg 范围内时与其峰面积成良好线性关系，检测低限为2.0μg/kg，3 个加标水平下回收率为76.0%～95.3%，相对标准偏差为5.4%～11.2%。该方法选择性好，灵敏度高，定性可靠，可满足苹果、山楂、番茄制品中棒曲霉素测定的要求。

High Performance Liquid Chromatography Tandem Mass Spectrometry Method for Determination of Patulin in Apple,Hawthorn and Tomato Products

A method was developed to clean-up and detect total patulin in of apple, hawthorn and tomato products using HPLCMS/ MS. Clear juice was diluted directly with acetonitrile and cleaned-up with MycoSep 228 multifunctional clean-up column. Cloudy juice or solid samples were pre-treated with pectinase, extracted with acetate ethyl, and then cleaned-up with MycoSep 228. Chromatographic separation was performed on a column Atlantis dC18 and determination was achieved by HPLC-MS/MS with ESI－(electronic spray ionization). The results demonstrated that the linear ranges are from 1.0 μg/kg to 200 μg/kg with the detection limit of 2.0 μg/kg. The average recoveries and the relative standard deviations range 76.0% to 95.3% and 5.4% to 11.2%. The method is simple, rapid, accurate and suitable for the quantification and confirmation determination of patulin in apples, hawthorn, tomato products.