桃果实成熟过程中活性氧和线粒体呼吸代谢相关酶的变化

以“雨花三号”水蜜桃为试样，研究了桃果实活性氧和线粒体呼吸代谢相关酶的变化对果实成熟衰老进程的影响。结果表明：桃果实成熟过程中硬度下降较快，呼吸强度有明显的高峰，并且果实硬度的快速变化期要明显早于呼吸高峰期。随着桃果实成熟衰老的加剧，果实内活性氧量有累积趋势。桃果实成熟过程中线粒体琥珀酸脱氢酶(SDH)、线粒体细胞色素氧化酶(CCO)、H+-ATPase酶和Ca2+-ATPase酶活性在成熟后期都有不同程度的降低，线粒体的钙含量却增大。活性氧代谢与线粒体内代谢联系紧密，它们是影响果实衰老的重要因素。

Changes of Active Oxygen and Mitochondria Respiratory Metabolism-related Enzymes during Maturation of Peach Fruit

The peach fruits of cultivar “Yuhua No. 3” were used to investigate the effects of changes of active oxygen and mitochondria respiratory metabolism-related enzymes on the maturation of peach fruit. Significant peaks of respiratory intensity lagged behind the rapid change of fruit firmness exist, and there is an accumulation trend in the content of active oxygen along with the intensification of peach fruit maturation. The results also showed that the content of Ca2+ in mitochondria increases during the maturation of peach fruits, while the activities of SDH, CCO, H+- ATPase and Ca2+-ATPase decrease at different degrees in the later period of maturation. It is preliminarily revealed that there is a close relationship between active oxygen metabolism and mitochondrial respiration, and both of them probably play an important role in the ripening and aging of peach fruit.