| 枯草芽孢杆菌B2(Bacillus subtilis B2)木聚糖酶在毕赤酵母中的表达 |
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| 引用本文： | 韩双艳,林小琼,张溪,林影.枯草芽孢杆菌B2(Bacillus subtilis B2)木聚糖酶在毕赤酵母中的表达[J].现代食品科技,2009,25(8). |
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| 作者姓名： | 韩双艳 林小琼 张溪 林影 |
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| 作者单位： | 华南理工大学生物科学与工程学院,广东,广州,510006 |
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| 基金项目： | 广东省工业科技攻关计划项目 |
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| 摘 要： | 木聚糖酶是主要工业用酶制剂之一,在食品行业应用广泛.将编码枯草芽孢杆菌(Bacillus subtilis B2)木聚糖酶XYN的基因克隆到pPIC9K载体中,构建了分泌型表达载体pPIC9K-XYN,载体经线性化后转化Pichia pastoris GS115,G筛选获得了分泌表达XYN的重组毕赤酵母工程菌GS115/pPIC9K-XYN.初步研究表明:以山毛榉木聚糖为底物时,重组毕赤酵母工程菌摇瓶水平发酵上清液中木聚糖酶酶活可迭1542.6 U/mL,重组木聚糖酶最适温度为50℃,最适pH值为6.0,并显示出较好的热稳定性和宽pH适应性.
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| 关 键 词： | 枯草芽孢杆菌 木聚糖酶 毕赤酵母 分泌表达 |
High level expression of Bacillus subtilis B2 Xylanase gene in Pichia pastoris |
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| HAN Shuang-yan,LIN Xiao-qiong,ZHANG Xi,LIN Ying.High level expression of Bacillus subtilis B2 Xylanase gene in Pichia pastoris[J].Modern Food Science & Technology,2009,25(8). |
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| Authors: | HAN Shuang-yan LIN Xiao-qiong ZHANG Xi LIN Ying |
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| Affiliation: | School of Bioscience and Bioengineering;South China University of Technology;Guangzhou 510006;China |
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| Abstract: | Xylanase is one of the important enzymes widely applied in food industries. The gene of Bacillus subtilis B2 xylanase was separated and cloned into the secreted expression vector pPIC9K to construct a recombinant plasmid pPIC9K-XYN. The plasmid was linearized and transformed into Pichia pastoris GS115 and Pichia pastoris recombinant strain GS115/pPIC9K-XYN was obtained. The xylanase activity of the culture supernatant of recombinant strain was up to 1542 U/mL towards beenchwood xylan. The optimal temperatur... |
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| Keywords: | Bacillus subtilis xylanase Pichia pastoris secreted expression |
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