Fibronectin modulates the morphology of osteoblast-like cells (MG-63) on nano-grooved substrates |
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Authors: | Wei-Bor Tsai Yen-Chung Ting Jung-Yen Yang Juin-Yih Lai Hsuan-Liang Liu |
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Affiliation: | (1) Department of Chemical Engineering, National Taiwan University, No. 1, Roosevelt Road, Sec 4, Taipei, Taiwan;(2) National Nano Device Laboratories, Hsinchu, Taiwan;(3) R&D Center for Membrane Technology and Department of Chemical Engineering, Chung Yuan Christian University, Chungli, Taoyuan, Taiwan;(4) Graduate Institute of Biotechnology and Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, Taipei, Taiwan |
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Abstract: | Cell interactions with biomaterials are affected by surface topographic and chemical cues. Although it is well-known that
nanometrical grooves/ridges structure modulates cellular spreading, elongation, and alignment, the combinational influence
of surface topographic and chemical cues is not well studied. In this study, nano-textured silicon substrata with parallel
ridges of 90, 250, or 500 nm wide, separated by grooves with equal width, were fabricated by electron beam lithography and
dry etching techniques. Osteoblast-like cells, MG-63, were cultured on the patterned substrata with or without pre-adsorption
of fibronectin. The cell morphology was imaged by scanning electron microscopy, and analyzed by image software. We found that
FN coating initially modulated cellular spreading, length, and orientation on all types of grooved surfaces. However, after
24 h of culture, the cell morphology was not affected by FN coating on the 250-nm and 500-nm surfaces, while FN decreased
cell alignment on the 90-nm surfaces. Our results suggest that surface chemical cues influence the initial cell-substratum
contact, while the long-term cellular morphology is dictated by surface topographic cues. |
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