| 高产刺糖菌素发酵菌株的选育 |
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| 引用本文： | 朱明军,江丹,胡飞,杜顺堂. 高产刺糖菌素发酵菌株的选育[J]. 农药, 2009, 48(8) |
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| 作者姓名： | 朱明军 江丹 胡飞 杜顺堂 |
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| 作者单位： | 1. 华南理工大学,生物科学与丁程学院,广州,510006
2. 华粤行有限公司生物制药部,广州,511442 |
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| 基金项目： | 广东省科技计划资助项目 |
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| 摘 要： | 采用紫外线结合氯化锂以及硫酸二乙酯结合氯化锂2种常规诱变方法对刺糖多孢菌进行复合诱变,分别筛选鼠李糖抗性突变株、2-脱氧-D-葡萄糖抗性突变株和刺糖菌素抗性突变株,并结合自然分离纯化,最终获得高产菌株D-S-23,其产量达N89.57 mg/L,较出发菌株提高了104.3%.
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| 关 键 词： | 刺糖菌素 选育 菌株 |
Breeding of Production Strains of Spinosads |
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| ZHU Ming-juna,JIANG Dana,HU Feib,DU Shun-tang. Breeding of Production Strains of Spinosads[J]. Pesticides, 2009, 48(8) |
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| Authors: | ZHU Ming-juna JIANG Dana HU Feib DU Shun-tang |
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| Affiliation: | 1.south china University of Technology a.school of Bioscience & Bioengineering;b.school of light industry & Food;guangzhou 510006;china;2.hua Yue enterprise holdings ltd.;guangzhou 511442;china |
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| Abstract: | The two routine methods of combined mutagenesis treatment,UV binding lithium chloride and diethyl sulfate binding lithium chloride,were used to mutagenize saccharopolyspora spinosa.some mutants resistant to rhamnose,2-deoxy-D-glucose and spinosad were screened respectively.Combining natural isolation and purification,mutant strain D-s-23 was selected.compared with the original strain,the titers of selected strain reached 89.57 mg/l,and the yield was increased by 104.3%. |
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| Keywords: | spinosad selection strain |
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