Effects of Cancer Presence and Therapy on the Platelet Proteome |
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| Authors: | Maudy Walraven Siamack Sabrkhany Jaco C. Knol Henk Dekker Inge de Reus Sander R. Piersma Thang V. Pham Arjan W. Griffioen Henk J. Broxterman Mirjam Oude Egbrink Henk M. W. Verheul Connie R. Jimenez |
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| Affiliation: | 1.Amsterdam UMC, Cancer Center Amsterdam, Vrije Universiteit Amsterdam, Medical Oncology, De Boelelaan 1117, 1081 HV Amsterdam, The Netherlands; (M.W.); (J.C.K.); (H.D.); (I.d.R.); (S.R.P.); (T.V.P.); (A.W.G.); (H.J.B.);2.Cardiovascular Research Institute Maastricht, Department of Physiology, Maastricht University Medical Center, 6229 ER Maastricht, The Netherlands; (S.S.); (M.O.E.) |
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| Abstract: | Platelets are involved in tumor angiogenesis and cancer progression. Previous studies indicated that cancer could affect platelet content. In the current study, we investigated whether cancer-associated proteins can be discerned in the platelets of cancer patients, and whether antitumor treatment may affect the platelet proteome. Platelets were isolated from nine patients with different cancer types and ten healthy volunteers. From three patients, platelets were isolated before and after the start of antitumor treatment. Mass spectrometry-based proteomics of gel-fractionated platelet proteins were used to compare patients versus controls and before and after treatment initiation. A total of 4059 proteins were detected, of which 50 were significantly more abundant in patients, and 36 more in healthy volunteers. Eight of these proteins overlapped with our previous cancer platelet proteomics study. From these data, we selected potential biomarkers of cancer including six upregulated proteins (RNF213, CTSG, PGLYRP1, RPL8, S100A8, S100A9) and two downregulated proteins (GPX1, TNS1). Antitumor treatment resulted in increased levels of 432 proteins and decreased levels of 189 proteins. In conclusion, the platelet proteome may be affected in cancer patients and platelets are a potential source of cancer biomarkers. In addition, we found in a small group of patients that anticancer treatment significantly changes the platelet proteome. |
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| Keywords: | platelets biomarkers cancer antitumor therapy proteomics |
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