| 绿豆酸性磷酸酶的分离纯化和部分性质研究 |
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| 引用本文： | 高亚朋,苏茉,梁建荣,黄洁,唐云明. 绿豆酸性磷酸酶的分离纯化和部分性质研究[J]. 中国粮油学报, 2011, 26(5): 92-96 |
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| 作者姓名： | 高亚朋 苏茉 梁建荣 黄洁 唐云明 |
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| 作者单位： | 西南大学生命科学学院重庆市甘薯工程研究中心三峡库区生态环境教育部重点实验室,重庆,400715 |
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| 摘 要： | 从绿豆芽中提取酸性磷酸酶粗酶液。经分段盐析,DEAE-琼脂糖柱离子交换层析及Superdex-200凝胶过滤后,得到经SDS-聚丙烯酰胺凝胶电泳为单一蛋白区带的酸性磷酸酶。提纯倍数为156.3,酶液比活力为347.0 U/mg。凝胶过滤法测定酶分子质量为34.6 ku,SDS-PAGE测定酶的亚基分子质量为33.1ku。证明绿豆酸性磷酸酶为单亚基酶。该酶最适pH为5.2 ku,最适温度为40℃。以pNPP作底物时Km为4.28×10-3mol/L。Mg2+、Co2+对酶有激活作用,而Hg2+,Cu2+,Pb2+和Zn2+对该酶有明显的抑制作用,依次为:Hg2+>Cu2+>Pb2+>Zn2+。K+,Ca2+对酶影响不明显。
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| 关 键 词： | 绿豆 酸性磷酸酶 分离纯化 性质 |
Study on Purification and Characterization of Acid Phosphatase from Mung Bean |
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| Gao Yapeng,Su Mo,Liang Jianrong,Huang Jie,Tang Yunming. Study on Purification and Characterization of Acid Phosphatase from Mung Bean[J]. Journal of the Chinese Cereals and Oils Association, 2011, 26(5): 92-96 |
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| Authors: | Gao Yapeng Su Mo Liang Jianrong Huang Jie Tang Yunming |
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| Affiliation: | Gao Yapeng Su Mo Liang Jianrong Huang Jie Tang Yunming(Key Laboratory of Eco-environments in Three Gorges Reservoir Region Ministry of Education Chongqing Sweetpotato Engineering Research Center,School of Life Science,Southwest University,Chongqing 400715) |
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| Abstract: | Acid Phosphatase(ACP) was extracted crudely from Mung Bean sprout.ACP was a single electrophoretic band in SDS-PAGE by ammonium sulfate fraction precipitation,DEAE-sepharose chromatography and Superdex S-200 gelfiltration.Its purification fold was 156.3,and the specific activity was 347.0 U/mg.Superdex S-200 chromatography showed that the molecular weight of this ACP was 34.6 kD,and SDS-PAGE showed that the molecular weight of ACP subunit was 33.1 kD.It also showed that ACP from Mung Bean was a single subun... |
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| Keywords: | Mung Bean acid phosphatase separation and purification characterization |
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