Osteoinduction by Functionally Graded Apatites of Bovine Origin Loaded with Bone Morphogenetic Protein-2 |
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| Authors: | Toshiyuki Akazawa Kohji Itabashi Masaru Murata Tomoya Sasaki Junichi Tazaki Makoto Arisue Masayoshi Kobayashi Tohru Kanno |
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| Affiliation: | Hokkaido Industrial Research Institute, Sapporo 060-0819, Japan; Second Department of Oral Surgery, School of Dentistry, Health Sciences University of Hokkaido, Ishikarigun 061-0293, Japan; Department of Chemical System Engineering, Kitami Institute of Technology, Kitami 090-8507, Japan |
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| Abstract: | Bioresorbable and functionally graded apatites (fg-HAp) ceramics, which are characterized by gradations in crystallinity and the grain size of hydroxyapatite (HAp:Ca10(PO4)6(OH)2), were designed using bovine bone by the calcination and partial dissolution–precipitation method. The fg-HAp ceramics had macropores of 100–600 μm originated from spongy bone, and micropores of 10–160 nm. Fg-HAp ceramics loaded with recombinant human bone morphogenetic protein-2 (rhBMP-2/fg-HAp) were implanted into the subcutaneous tissue of rats. Eight weeks after the implantation, the surface and bulk degradations of the fg-HAp-containing body fluid occured, and small pieces of fg-HAp were incorporated into the induced bone and fatty marrow, suggesting that osteoinduction occurred in conjunction with bone remodeling. The rhBMP-2/fg-HAp ceramics developed could become a resorbable biomimetic material with fast bioresorption and osteoinduction characteristics. |
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